Genistein-induced cell cycle arrest and apoptosis in a head and neck squamous cell carcinoma cell line
Genistein-induced cell cycle arrest and apoptosis in a head and neck squamous cell carcinoma cell line
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DOI：
10.1207/s15327914nc340102
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发表时间：
1999-01-01
影响因子：
2.9
通讯作者：
Sarkar, FH
中科院分区：
文献类型：
--
作者：
Alhasan, SA;Pietrasczkiwicz, H;Sarkar, FH
流行病学研究表明，亚洲国家，特别是中国和日本的乳腺癌、前列腺癌和结肠癌发病率低于美国。据信，染料木黄酮，一种天然酪氨酸激酶抑制剂和所述产物的代谢物，可能负责保护免受这些癌症。染料木黄酮在乳腺癌、前列腺癌和Jurkat T细胞白血病细胞系中显示出抑制细胞增殖并诱导细胞周期停滞在G（2）-M期。然而，此类研究尚未在头颈部鳞状细胞癌中报道。本文报道了染料木黄酮对人鳞状细胞癌细胞株HN 4增殖的抑制作用。此外，染料木黄酮导致细胞周期停滞在S/G（2）-M期，并诱导这些细胞的程序性细胞死亡（凋亡）。这些作用似乎是剂量和时间依赖性的，不可逆的，当细胞在无染料木黄酮的培养基中再培养长达72小时时持续存在，并且特异性远离肿瘤细胞，因为染料木黄酮不影响正常的角质形成细胞。这些结果表明，如果染料木黄酮在临床试验中显示出类似的结果，它可以是一个潜在的化学预防/化疗剂的头部和颈部的癌症。
Epidemiological studies have shown a lower incidence of breast, prostate, and colon cancers in Asian countries, particularly China and Japan, than in the United States. It is believed that genistein, a natural tyrosine kinase inhibitor and a metabolite of say products, may be responsible for the protection from these cancers. Genistein was shown to inhibit cell proliferation and to induce cell cycle arrest at the G(2)-M phase in breast, prostate, and jurkat T cell leukemia cell lines. However such studies have not been reported in squamous cell cancers of the head and neck. In this report, lye show that genistein inhibits proliferation of a squamous cell carcinoma cell line HN4. Additionally genistein caused cell cycle arrest at the S/G(2)-M phase and induced programmed cell death (apoptosis) in these cells. These effects appear to be dose and time dependent, irreversible, persisting when the cells were recultured in genistein-free medium for up to 72 hours, and specific far tumor cells, because genistein did not affect normal keratinocytes. These results suggest that if genistein shows similar results in clinical trials, it can be a potential chemopreventive/chemotherapeutic agent for cancers of the head and neck.