Effects of the hook of Uncaria rhynchophylla on neurotoxicity in the 6-hydroxydopamine model of Parkinson's disease
Effects of the hook of Uncaria rhynchophylla on neurotoxicity in the 6-hydroxydopamine model of Parkinson's disease
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DOI：
10.1016/j.jep.2009.08.023
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发表时间：
2009-11-12
影响因子：
5.4
通讯作者：
Oh, Myung Sook
中科院分区：
文献类型：
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作者：
Shim, Jin Sup;Kim, Hyo Geun;Oh, Myung Sook
民族药理学相关性：虽然钩藤（URH）的钩子是在东北亚用于治疗帕金森病（PD）样症状如震颤的传统草药，但它尚未在PD模型中进行实验评估。研究目的：我们研究了URH对6-羟基多巴胺（6-OHDA）诱导的体外和体内PD模型神经毒性的影响。材料和方法：在6-OHDA应激的PC 12细胞中，使用3-（4，5-二甲基噻唑-2-基）-2，5-二苯基-四唑溴化物、活性氧（ROS）、总谷胱甘肽（GSH）和半胱天冬酶-3测定来评估URH（URE）的水提取物的细胞活力、抗氧化活性和抗凋亡活性。我们还研究了行为恢复和多巴胺能神经元的保护，使用阿朴吗啡诱导的旋转试验和酪氨酸羟化酶免疫组化在偏侧帕金森病大鼠模型的单侧6-OHDA病变的内侧前脑bundle.Results：在PC 12细胞，URE显着减少细胞死亡和ROS的产生，增加GSH水平，并抑制caspase-3活性诱导的6-OHDA。在6-OHDA损伤的大鼠，后处理与URE（5 mg/kg/天，14天）显着减少阿扑吗啡诱导的旋转，并降低多巴胺能神经元的损失，在黑质parsaparta.Conclusions：在PD模型中，通过抗氧化和抗凋亡活性，URE具有对6-OHDA诱导的毒性的神经保护活性。(C)2009爱思唯尔爱尔兰有限公司保留所有权利。
Ethnopharmacological relevance: While the hook of Uncaria rhynchophylla (URH) is a traditional herb used in northeast Asia for the treatment of Parkinson's disease (PD)-like symptoms such as tremor, it has not been experimentally evaluated in a PD model.Aim of the study: We investigated the effects of URH on 6-hydroxydapamine (6-OHDA)-induced neurotoxicity in in vitro and in vivo models of PD.Materials and methods: The cell viability, anti-oxidative activity, and anti-apoptotic activity of a water extract of URH (URE) were assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide, reactive oxygen species (ROS), total glutathione (GSH), and caspase-3 assays in PC12 cells stressed by 6-OHDA. We also investigated the behavioral recovery and dopaminergic neuron protection of URE using an apomorphine-induced rotation test and tyrosine hydroxylase immunohistochemistry in the hemi-parkinsonian rat model of the unilateral 6-OHDA lesion of the medial forebrain bundle.Results: In PC12 cells, URE significantly reduced cell death and the generation of ROS, increased GSH levels, and inhibited caspase-3 activity induced by 6-OHDA. In 6-OHDA-lesioned rats, posttreatment with URE (5 mg/kg/day for 14 days) significantly reduced apomorphine-induced rotation, and it lowered dopaminergic neuronal loss in substantia nigra pars compacta.Conclusions: URE possesses neuroprotective activity against 6-OHDA-induced toxicity through antioxidative and anti-apoptotic activities in PD models. (C) 2009 Elsevier Ireland Ltd. All rights reserved.