Complement analysis in the 21st century
Complement analysis in the 21st century
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DOI：
10.1016/j.molimm.2007.06.150
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发表时间：
2007-09-01
影响因子：
3.6
通讯作者：
Kirschfink, Michael
中科院分区：
文献类型：
--
作者：
Mollnes, Tom Eirik;Jokiranta, T. Sakari;Kirschfink, Michael
临床中的补体分析通常与C3和C4的定量、C1抑制剂的测量和补体活性的筛查相关。这些分析已经在常规诊断实验室中使用了几十年。然而，近年来，补体分析的领域已经大大扩展，引入了新的测定来检测补体活化产物，并进一步扩展到遗传分析，以揭示补体缺陷的基础并鉴定与确定的疾病如非典型溶血性尿毒症综合征和年龄相关性黄斑变性相关的突变和多态性。在这里，我们回顾了补体分析的现状，包括补体活性和补体激活产物的定量测定，以及检测缺陷，突变和多态性的遗传方法。这是一个最近取得重大进展的领域，与补体在人类疾病中的作用的研究进展平行。然而，显然需要对分析方法达成共识和标准化。这将是未来补体社会面临的一个重大挑战。(c)2007爱思唯尔有限公司保留所有权利。
Complement analysis in the clinic is usually associated with the quantification of C3 and C4, measurement of C1-inhibitor and screening for complement activity. These analyses have been available in routine diagnostic laboratories for decades. In recent years, however, the field of complement analysis has expanded considerably, with the introduction of novel assays to detect complement activation products, and spreading still further towards genetic analysis to reveal the basis of complement deficiencies and identify mutations and polymorphisms associated with defined diseases such as atypical haemolytic uraemic syndrome and age related macular degeneration. Here we review the current status of complement analysis, including assays for the quantification of complement activity and complement activation products, together with genetic methods for the detection of deficiencies, mutations and polymorphisms. This is an area where significant developments have been made recently, paralleling the research advances into the role of complement in human disease. It is clear, however, that there is a need for consensus and standardisation of analytical methods. This will be a major challenge for the complement society in the future. (c) 2007 Elsevier Ltd. All rights reserved.