Multiple Factors Determine the Structure of Bacterial Communities Associated With Aedes albopictus Under Artificial Rearing Conditions
Multiple Factors Determine the Structure of Bacterial Communities Associated With Aedes albopictus Under Artificial Rearing Conditions
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DOI：
10.3389/fmicb.2020.00605
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发表时间：
2020-04-15
影响因子：
5.2
通讯作者：
Bourtzis, Kostas
中科院分区：
文献类型：
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作者：
Chen, Shi;Zhang, Dongjing;Bourtzis, Kostas
昆虫共生体是寄主行为的主要操纵者。它们对生殖力、雄性交配竞争力和一般生物质量等参数的影响，对昆虫不育技术（SIT）的有效性有重大影响。SIT目前正在开发和应用于人类疾病载体，包括Ae。白纹伊蚊作为一种环境友好的种群抑制方法，因此，对肠道微生物群的表征及其在人工饲养中的利用都有新的兴趣。在本研究中，细菌群落的卵，幼虫，和成人（男性和女性）的人工饲养的Ae。白纹伊蚊，使用培养依赖性和培养非依赖性方法表征。从幼虫食物和糖溶液（成虫食物）中分别分离出对应于13个和5个细菌属的蚊子相关细菌。雌性的共生体群落受到提供血餐的影响。假单胞菌属和肠杆菌属的引入或增强与血粉，而沙雷氏菌属是相对稳定的成年期的女性。这些类群在雌性肠道中的维持可能与血液消化有关。男性和女性的肠道相关微生物群是不同的，在出现后早期开始，并在较老的阶段继续。我们的研究结果表明，鸡蛋含有超过15个属的细菌，包括芽孢杆菌，金黄杆菌，和土方志贺菌，这也是女性成年人的肠道菌群的主要组成部分，在吸血前后，表明潜在的世代之间的传播。我们的研究结果提供了一个彻底的研究，鸡蛋和肠道相关的细菌的人工饲养。白纹伊蚊，这可能是重要的进一步研究使用益生菌，以提高蚊子人工饲养和SIT应用的有效性。
Insect symbionts are major manipulators of host's behavior. Their effect on parameters such as fecundity, male mating competitiveness, and biological quality in general, can have a major influence on the effectiveness of the sterile insect technique (SIT). SIT is currently being developed and applied against human disease vectors, including Ae. albopictus, as an environment-friendly method of population suppression, therefore there is a renewed interest on both the characterization of gut microbiota and their exploitation in artificial rearing. In the present study, bacterial communities of eggs, larvae, and adults (both males and females) of artificially reared Ae. albopictus, were characterized using both culture-dependent and culture-independent approaches. Mosquito-associated bacteria corresponding to thirteen and five bacteria genera were isolated from the larval food and the sugar solution (adult food), respectively. The symbiont community of the females was affected by the provision of a blood meal. Pseudomonas and Enterobacter were either introduced or enhanced with the blood meal, whereas Serratia were relatively stable during the adult stage of females. Maintenance of these taxa in female guts is probably related with blood digestion. Gut-associated microbiota of males and females were different, starting early after emergence and continuing in older stages. Our results indicate that eggs contained bacteria from more than fifteen genera including Bacillus, Chryseobacterium, and Escherichia-Shigella, which were also main components of gut microbiota of female adults before and after blood feeding, indicating potential transmission among generations. Our results provided a thorough study of the egg- and gut-associated bacteria of artificially reared Ae. albopictus, which can be important for further studies using probiotic bacteria to improve the effectiveness of mosquito artificial rearing and SIT applications.