Formation of Ring-Shaped Microtubule Assemblies through Active Self-Organization on Dynein
Formation of Ring-Shaped Microtubule Assemblies through Active Self-Organization on Dynein
复制标题
通过动力蛋白主动自组织形成环形微管组件
DOI：
10.1038/pj.2013.89
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发表时间：
2013
期刊：
影响因子：
2.8
通讯作者：
Akira Kakugo
中科院分区：
文献类型：
--
作者：
Masaki Ito;Arif Md Rashedul Kabir;Daisuke Inoue;Takayuki Torisawa;Yoko Toyoshima;Kazuki Sada;Akira Kakugo
微管-驱动蛋白是一种生物分子马达系统，是构建人工生物机械的理想材料。一个积极的自组织（AcSO）的方法，涉及一个特定的链霉亲和素（St）-生物素（Bt）的相互作用已被开发组装成一个高度有序的结构，通过利用他们的运动性的驱动蛋白包被的表面上的MT。动力蛋白是另一种生物分子马达，其在与驱动蛋白相反的方向上沿着MT沿着移动。动力蛋白尚未用于证明MT的AcSO。在这项研究中，我们报告了第一次成功的演示的AcSO的MT的动力蛋白涂层的表面上产生环形MT组件类似的驱动蛋白。我们发现，环状的动力蛋白MT组件显示出相等的顺时针和逆时针旋转运动。这项工作将丰富的积木设计面向未来的运动蛋白为基础的人工设备。
Microtubule (MT)–kinesin, a biomolecular motor system, is a promising candidate for construction of artificial biomachines for a variety of nanotechnology applications. An active self-organization (AcSO) method involving a specific streptavidin (St)–biotin (Bt) interaction has been developed to assemble MTs into a highly ordered structure by exploiting their motility on a kinesin-coated surface. Dynein is another biomolecular motor that moves along the MTs in the opposite direction from kinesin. Dynein has not yet been used to demonstrate the AcSO of MTs. In this study, we report the first successful demonstration of the AcSO of MTs on a dynein-coated surface to produce ring-shaped MT assemblies similar to those of kinesin. We found that ring-shaped MT assemblies obtained on dynein showed equal clockwise and counterclockwise rotational motion. This work will enrich the building blocks for designing future oriented motor protein-based artificial devices.