Comparison of lipopolysaccharide structures of Bordetella pertussis clinical isolates from pre- and post-vaccine era
Comparison of lipopolysaccharide structures of Bordetella pertussis clinical isolates from pre- and post-vaccine era
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DOI：
10.1016/j.carres.2013.05.002
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发表时间：
2013-08-30
影响因子：
3.1
通讯作者：
Caroff, Martine
中科院分区：
文献类型：
--
作者：
AlBitar-Nehme, Sami;Basheer, Soorej M.;Caroff, Martine
内毒素是脂多糖（LPS），是革兰氏阴性菌外膜的主要成分。百日咳博德泰拉脂多糖是百日咳唯一的主要抗原，尚未对疫苗接种前后的分离株进行分析。我们在此比较了4个临床分离株与疫苗株BP 1414的LPS结构。所有的理化分析，包括SDS-PAGE， TLC和不同的MALDI质谱分析方法是收敛的。他们帮助证明，与其他一些百日咳主要抗原相反，在十二糖核心结构以及整个LPS分子中没有发生修饰。这些结果表明这些主要抗原是良好的潜在疫苗成分。这种保守LPS结构的分子模型也证实了先前实验的结论，从而产生了抗LPS单克隆抗体，并确定了这些主要抗原的主要表位。(C) 2013 Elsevier Ltd.版权所有。
Endotoxins are lipopolysaccharides (LPS), and major constituents of the outer membrane of Gram-negative bacteria. Bordetella pertussis LPS were the only major antigens, of this agent of whooping-cough, that were not yet analyzed on isolates from the pre-and post-vaccination era. We compared here the LPS structures of four clinical isolates with that of the vaccine strain BP 1414. All physico-chemical analyses, including SDS-PAGE, TLC, and different MALDI mass spectrometry approaches were convergent. They helped demonstrating that, on the contrary to some other B. pertussis major antigens, no modification occurred in the dodecasaccharide core structure, as well as in the whole LPS molecules. These results are rendering these major antigens good potential vaccine components. Molecular modeling of this conserved LPS structure also confirmed the conclusions of previous experiments leading to the production of anti-LPS monoclonal antibodies and defining the main epitopes of these major antigens. (C) 2013 Elsevier Ltd. All rights reserved.