NONINDUCED CYCLIC HYDROXAMIC ACIDS IN WHEAT DURING JUVENILE STAGE OF GROWTH
NONINDUCED CYCLIC HYDROXAMIC ACIDS IN WHEAT DURING JUVENILE STAGE OF GROWTH
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DOI：
10.1016/0031-9422(94)00831-d
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发表时间：
1995-04-01
期刊：
影响因子：
3.8
通讯作者：
IWAMURA, H
中科院分区：
文献类型：
--
作者：
NAKAGAWA, E;AMANO, T;IWAMURA, H
发芽小麦中存在2，4-二羟基-1，4-苯并恶嗪-3-酮葡萄糖甙（DIBOA-G）及其甲氧基类似物2，4-二羟基-7-甲氧基-1，4-苯并恶嗪-3-酮葡萄糖甙（DIMBOA-G）;萌发后不久就出现相应的苷元DIBOA和DIMBOA。这些化合物的量在发芽后12-48小时达到最大值，然后随着植物开始自养生长而降低至不可检测的水平。它们出现的时间几乎不受使用消毒或未消毒的种子，感染病原体和用刀片受伤的影响。发现DIBOA的浓度为0.2-0.3 nmol mg（-1）fr. wt（如果假定植物组织的密度是均匀和统一的，则为0.2-0.3 mM），而DIMBOA的浓度为0.7-1.0 nmol mg（-1）（0.7-1.0 mM）。糖苷配基延缓芽管生长的真菌种在0.3毫米。这些观察表明，苯并恶嗪酮类的外观是作为防御化合物在少年阶段的增长。[C-14]邻氨基苯甲酸在给予从出苗前种子分离的胚时掺入DIBOA-G和DIMBOA-G中，表明该系列化合物是通过从头合成产生的。
2,4-Dihydroxy-1,4-benzoxazine-3-one glucoside (DIBOA-G) and its methoxy analogue, 2,4-dihydroxy-7-methoxy-1,4-benzoxazine-3-one glucoside (DIMBOA-G), were present in germinating wheat (Triticum aestivum); the corresponding aglycones, DIBOA and DIMBOA, appeared soon after germination. The amounts of these compounds reached a maximum 12-48 hours after germination, and then decreased to undetectable levels as the plants began autotrophic growth. The time of their appearance was little affected by using seeds either sterilized or non-sterilized, by infection with pathogens and wounding with a razor blade. The concentration of DIBOA was found to be 0.2-0.3 nmol mg(-1) fr. wt (0.2-0.3 mM if the density of plant tissue is assumed to be uniform and unity) and that of DIMBOA was 0.7-1.0 nmol mg(-1) (0.7-1.0 mM). The aglycones retarded the germ tube growth of species of fungi at 0.3 mM. These observations suggest that the appearance of benzoxazinones is as defence compounds in the juvenile stage of growth. [C-14]Anthranilic acid was incorporated into DIBOA-G and DIMBOA-G when administered to embryos isolated from pre-emerging seeds, showing that the series of compounds are generated by de novo synthesis.