Metabolism of Reactive Oxygen Species in Cytoplasmic Male Sterility of Rice by Marking Upmost Pulvinus Interval
Metabolism of Reactive Oxygen Species in Cytoplasmic Male Sterility of Rice by Marking Upmost Pulvinus Interval
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DOI：
10.1007/s12010-014-1346-8
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发表时间：
2015-02-01
影响因子：
3
通讯作者：
Huang, Qunce
中科院分区：
文献类型：
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作者：
Li, Jianxin;Dai, Ximei;Huang, Qunce
植物细胞内活性氧（ROS）和丙二醛（MDA）的过量积累是细胞凋亡的重要诱导因子。为阐明水稻细胞质雄性不育（CMS）丙二醛（MDA）产生与清除的代谢机理，以不育系和保持系为材料，采用标记最高叶枕间隔的试验方法，研究了不同发育时期MDA产生与清除的关系。结果表明，在五个生育期内，不育系穗部和叶片中MDA含量均显著高于保持系（p < 0.05）。在5个时期中，不育系穗位旗叶在距离0 mm时期（减数分裂期）的MDA含量最高。不育穗和叶片中MDA含量的增加对超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和过氧化物酶（POD）的酶活性有诱导作用。但在败育高峰期，MDA过量积累，抗氧化酶活性显著降低，导致MDA的产生和清除失衡。
Reactive oxygen species (ROS) and malondialdehyde (MDA) in plant cell are thought to be important inducible factors of cell apoptosis if excessively accumulated in cells. To elucidate the metabolic mechanism of MDA production and scavenging in the cytoplasmic male-sterile (CMS) rice, CMS line and maintainer were employed for studying the relationship at different developmental stages by marking upmost pulvinus interval method of experiment. The results showed that the panicles and leaves of the CMS line had a noticeable higher MDA content than those of maintainer line at all five stages that had been investigated (p < 0.05). MDA content in the CMS line in the flag leaves of auricle in the distance 0 mm stage (the meiosis stage) was the highest of the five stages. The increase of MDA contents in sterile panicles and leaves had inducible effects on the enzymic activity of superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD). However, at the abortion peak stage, MDA was excessively accumulated and antioxidant enzymic activity reduced significantly, resulting in the generation and scavenging of MDA out of balance.