THE AGGLUTINATION OF BETA-HEMOLYTIC STREPTOCOCCI BY LECTINS
THE AGGLUTINATION OF BETA-HEMOLYTIC STREPTOCOCCI BY LECTINS
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DOI：
10.1099/00222615-39-6-440
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发表时间：
1993-12-01
影响因子：
3
通讯作者：
STOBBERINGH, EE
中科院分区：
文献类型：
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作者：
KELLENS, JTC;JACOBS, JA;STOBBERINGH, EE
研究了从不同植物和真菌中分离的25种凝集素对95株临床分离的溶血性链球菌的凝集能力。细胞悬液未经处理、胰蛋白酶处理或在pH 2.0下煮沸。95个未经处理的细胞悬浮液与任何凝集素都没有明显的反应。当胰蛋白酶化细胞时，42株细胞被一种或多种凝集素凝集，在pH 2煮沸后，所有菌株都被凝集。经胰蛋白酶处理后，观察到20种不同的凝集模式，煮沸后观察到19种凝集模式，其中4种相似。在兰斯菲尔德C组和其中一些模式之间发现了相关性。有些凝集素与C群链球菌有特异性反应；DBA和WFA均对D-GalNAc、DSA （glcnac特异性凝集素）和RPA具有复杂的特异性，仅对C组菌株起反应。此外，仅与50%的B组链球菌发生反应的是黑金玛奇菌凝集素。凝集素凝集试验可重复性好，操作简单，相对便宜，因此适用于β -溶血链球菌细胞壁结构和流行病学的研究。
The ability of 25 lectins, isolated from different plants and fungi, to agglutinate 95 clinical isolates of beta-haemolytic streptococci was examined. Cell suspensions were untreated, trypsin-treated or boiled at pH 2.0. None of the 95 untreated cell suspensions gave a visible reaction with any of the lectins. When the cells were trypsinised, 42 strains were agglutinated with one or more lectin and after boiling at pH 2, all the strains were agglutinated. After treatment with trypsin, 20 different agglutination patterns were observed, and after boiling, 19 patterns, four of which were similar. A correlation was found between Lancefield group C and some of these patterns. Some lectins reacted specifically with group C streptococci; DBA and WFA, both specific for D-GalNAc, DSA, a GlcNAc-specific lectin, and RPA, which showed a complex specificity, reacted only with group C strains. Furthermore, the lectin of Maackia amurensis reacted with 50 % of group B streptococci only. Agglutination assays with lectins were reproducible, easy to perform, relatively inexpensive and, therefore, applicable to studies of cell-wall structure and epidemiology of beta-haemolytic streptococci.