In-Depth Proteomic Map of Innate Lymphoid Cells from Healthy Human Skin and Blood.
In-Depth Proteomic Map of Innate Lymphoid Cells from Healthy Human Skin and Blood.
复制标题
健康人类皮肤和血液先天淋巴细胞的深入蛋白质组图谱。
DOI：
10.1016/j.jid.2023.07.011
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发表时间：
2024
期刊：
影响因子：
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通讯作者：
Dyring-Andersen,Beatrice
中科院分区：
文献类型：
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作者：
Teunissen,MarcelBM;PilgaardMøller,LineB;Løvendorf,MarianneB;Skov,Lone;Bonefeld,CharlotteM;Bekkenk,MarcelW;Clark,RachaelA;Mann,Matthias;Dyring-Andersen,Beatrice
先天淋巴样细胞（ilc）在皮肤相关免疫系统中扮演着重要的角色，然而人们对其蛋白质组学和蛋白质组学多样性知之甚少。在这项研究中，我们使用最先进的蛋白质组学技术描述了来自健康人类皮肤和血液的ILC2s和ILC3s组成性表达的约6600种蛋白质。尽管绝大多数的蛋白质都是由两个ILC亚群和两个腔室表达的，但皮肤的ILC2s和ILC3s比血液中的ILC3s更明显。只有皮肤ILC3s表达了唯一检测到的蛋白。我们深入的蛋白质组学数据集使我们能够定义ILC亚群的分化标记谱簇，探索已知具有免疫功能的蛋白质的分布和丰度，以及鉴定以前未涉及ILC生物学的亚群特异性蛋白质。综上所述，我们的分析大大扩展了对ILC亚群蛋白表达特征的理解。展望未来，这些蛋白质组学数据集将为未来ILC生物学研究提供宝贵的资源。
Innate lymphoid cells (ILCs) are essential players in the skin-associated immune system, nevertheless little is known about their proteomes and proteomic diversity. In this study, we describe about 6,600 proteins constitutively expressed by ILC2s and ILC3s from healthy human skin and blood using state-of-the-art proteomics. Although the vast majority of proteins was expressed by both ILC subsets and in both compartments, the skin ILC2s and ILC3s were more distinct than their counterparts in blood. Only skin ILC3s expressed uniquely detected proteins. Our in-depth proteomic dataset allowed us to define the cluster of differentiation marker profiles of the ILC subsets, explore distribution and abundance of proteins known to have immunological functions, as well as identify subset-specific proteins that have not previously been implicated in ILC biology. Taken together, our analyses substantially expand understanding of the protein expression signatures of ILC subsets. Going forward, these proteomic datasets will serve as valuable resources for future studies of ILC biology.