Diamond like carbon coated films for enzyme electrodes; characterization of biocompatibility and substrate diffusion limiting properties
Diamond like carbon coated films for enzyme electrodes; characterization of biocompatibility and substrate diffusion limiting properties
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用于酶电极的类金刚石碳涂层薄膜；
DOI：
10.1016/0003-2670(94)00380-5
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发表时间：
1995
影响因子：
6.2
通讯作者：
P. Vadgama
中科院分区：
文献类型：
--
作者：
S. Higson;P. Vadgama
研究了一系列类金刚石（DLC）涂层微孔聚碳酸酯和DLC涂层透析（血液透析）膜的生物相容性和基材扩散限制性能。该表征建立在先前发现的基础上，其中DLC涂覆的膜赋予增强的酶电极性能。在这项研究中，电极的线性范围已从10 mM葡萄糖（0.01 μm孔径膜）扩展到160 mM。这些发现与DLC沉积的持续时间和葡萄糖渗透性的相关降低相关。微孔膜和透析膜的渗透系数比也被发现是重要的，低葡萄糖/O2渗透率比赋予葡萄糖线性响应范围的扩展。酶电极中使用的DLC涂层膜也显示出增强的血液相容性，如通过扫描电子显微镜检查的血液成分的灵敏度变化和表面沉积所确定的。在传感器响应于生物污损/工作电极钝化过程中的减少的损失与DLC涂层膜可能赋予酶电极性能的扩展的线性范围之间进行相关性。具体参考全血内葡萄糖水平的测定。
The biocompatibility and substrate diffusion limiting properties for a range of diamond like carbon (DLC) coated microporous polycarbonate and DLC coated dialysis (haemodialysis) membranes have been studied. This characterisation builds upon previous findings where DLC coated membranes imparted enhanced enzyme electrode performance. In this study electrode linear ranges have been extended from 10 mM glucose for a 0.01 μm pore size membrane to 160 mM. These findings correlated with the duration of DLC deposition and associated reductions in permeability for glucose. Permeability coefficient ratios for both microporous and dialysis membranes were also found to be important with low glucose/O2permeability ratios imparting extensions in glucose linear response range. DLC coated membranes employed within enzyme electrodes have also been shown to exhibit enhanced haemocompatibility as determined by both sensitivity change and surface deposition of blood components examined by scanning electron microscopy. Correlations are made between the reduced losses in sensor response to biofouling/ working electrode passivation processes, and extended linear ranges that DLC coated membranes may impart to enzyme electrode performance. Particular reference is made to the determination of glucose levels within whole blood.