Increased expression of FcgammaRI/CD64 on circulating monocytes parallels ongoing inflammation and nephritis in lupus.
Increased expression of FcgammaRI/CD64 on circulating monocytes parallels ongoing inflammation and nephritis in lupus.
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DOI：
10.1186/ar2590
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发表时间：
2009
影响因子：
4.9
通讯作者：
Richards HB
中科院分区：
文献类型：
--
作者：
Li Y;Lee PY;Sobel ES;Narain S;Satoh M;Segal MS;Reeves WH;Richards HB
IgG Fcγ/CD 64的高亲和力受体在狼疮性肾炎（LN）的发生发展中至关重要。通过含IgG的免疫复合物交联募集的单核细胞上的Fc受体是免疫复合物介导的系统性红斑狼疮（SLE）肾炎的关键步骤。本研究的目的是确定Fc受体（FcγR）I在循环单核细胞上的表达是否与SLE患者的全身炎症和肾脏疾病相关。我们研究了205例SLE患者（132例LN和73例无LN）沿着74例健康对照者。通过流式细胞术评价了CD 14（单核细胞）、FcγRI/CD 64、FcγRII/CD 32和FcγRIII/CD 16的表面表达。通过测定迁移能力和产生CCL 2（单核细胞趋化蛋白1）的能力来评估单核细胞功能。采用散射比浊法测定超敏C反应蛋白、补体C3、C4。SLE患者外周血单核细胞表面FcγRIII/CD 16和FcγRIII/CD 32的表达与对照组相比无显著性差异。而FcγRI/CD 64在SLE患者中的表达明显高于LN患者。FcγRI/CD 64表达与血清肌酐和全身炎症指标呈正相关。FcγRI/CD 64高表达患者的单核细胞也表现出趋化性和产生单核细胞趋化蛋白1的能力增加。SLE患者循环单核细胞FcγRI/CD 64表达增加与全身炎症和肾脏疾病相关。我们认为，SLE患者外周血单核细胞被免疫复合物和/或促炎介质激活，上调FcγRI/CD 64的表面表达。活化单核细胞的趋化和炎症潜能增强可能参与了SLE免疫细胞募集和肾损伤的恶性循环。
The high-affinity receptor for IgG Fcγ/CD64 is critical for the development of lupus nephritis (LN). Cross-linking Fc receptor on recruited monocytes by IgG-containing immune complexes is a key step in immune-complex-mediated nephritis in systemic lupus erythematosus (SLE). The goal of this study was to determine whether expression of Fc receptor (FcγR) I on circulating monocytes is associated with systemic inflammation and renal disease in SLE patients. We studied 205 SLE patients (132 with LN and 73 without LN) along with 74 healthy control individuals. Surface expression of CD14 (monocytes), FcγRI/CD64, FcγRII/CD32, and FcγRIII/CD16 was evaluated by flow cytometry. Monocyte function was assessed by determining the migratory capacity and the ability to produce CCL2 (monocyte chemotractic protein 1). High-sensitivity C-reactive protein, C3 and C4 were measured by nephelometry. There was little difference in the expression of FcγRIII/CD16 or FcγRIII/CD32 on circulating monocytes between patients with SLE and control individuals. In contrast, FcγRI/CD64 expression was significantly higher in SLE patients and even higher in patients with LN. FcγRI/CD64 expression was positively associated with serum creatinine and indicators of systemic inflammation. Monocytes from patients with high FcγRI/CD64 expression also exhibited increased chemotaxis and capacity to produce monocyte chemotractic protein 1. Increased FcγRI/CD64 expression on circulating monocytes parallels systemic inflammation and renal disease in SLE patients. We propose that circulating monocytes activated by immune complexes and/or proinflammatory mediators upregulate surface expression of FcγRI/CD64 in SLE. The enhanced chemotactic and inflammatory potential of the activated monocytes may participate in a vicious cycle of immune cell recruitment and renal injury in SLE.
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影响因子：
6.1
作者：
Mozo, L;Suárez, A;Gutiérrez, C
通讯作者：
Gutiérrez, C
DOI：
10.1084/jem.185.7.1371
发表时间：
1997-04-07
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Lloyd CM;Minto AW;Dorf ME;Proudfoot A;Wells TN;Salant DJ;Gutierrez-Ramos JC
通讯作者：
Gutierrez-Ramos JC
影响因子：
--
作者：
Nacionales, Dina C.;Kelly-Scumpia, Kindra M.;Reeves, Westley H.
通讯作者：
Reeves, Westley H.
DOI：
10.1084/jem.189.1.179
发表时间：
1999-01-04
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Clynes R;Maizes JS;Guinamard R;Ono M;Takai T;Ravetch JV
通讯作者：
Ravetch JV
DOI：
10.1084/jem.20051503
发表时间：
2006-09-04
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Mackay M;Stanevsky A;Wang T;Aranow C;Li M;Koenig S;Ravetch JV;Diamond B
通讯作者：
Diamond B