Fabrication and endothelialization of collagen-blended biodegradable polymer nanofibers: Potential vascular graft for blood vessel tissue engineering
Fabrication and endothelialization of collagen-blended biodegradable polymer nanofibers: Potential vascular graft for blood vessel tissue engineering
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DOI：
10.1089/ten.2005.11.1574
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发表时间：
2005-09-01
影响因子：
--
通讯作者：
Ramakrishna, S
中科院分区：
文献类型：
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作者：
He, W;Yong, T;Ramakrishna, S
静电纺丝胶原蛋白共混的聚乳酸-聚己内酯共聚物[ P（LLA-CL），70：30]复合物在形态和化学上均能模拟细胞外基质，在组织工程中具有巨大的应用潜力。通过静电纺丝制备了具有不同聚合物与胶原重量比的共混纳米纤维。通过扫描电子显微镜和透射电子显微镜研究了共混纳米纤维的外观。纳米纤维表现出光滑的表面和窄的直径分布，其中60%的纳米纤维的直径在100和200 nm之间。衰减全反射-傅里叶变换红外光谱和X射线光电子能谱证实了胶原分子在纳米纤维表面的存在。将人冠状动脉内皮细胞（HCAEC）接种到混合纳米纤维上进行活力、形态发生、附着和表型研究。采用逆转录-聚合酶链反应技术研究了5种内皮细胞特征性标志物，包括4种细胞粘附分子和1种内皮细胞偏好基因（von Willebrand因子）。结果表明，胶原蛋白共混的聚合物纳米纤维可以提高HCAEC的活力，铺展和附着，而且，保持EC表型。共混静电纺丝技术显示出通过添加各种成分（例如，例如，在一个实施例中，生长因子）来构建组织工程支架，特别是血管工程支架。
Electrospun collagen-blended poly( L-lactic acid)-co-poly( epsilon-caprolactone) [ P( LLA-CL), 70: 30] nanofiber may have great potential application in tissue engineering because it mimicks the extracellular matrix ( ECM) both morphologically and chemically. Blended nanofibers with various weight ratios of polymer to collagen were fabricated by electrospinning. The appearance of the blended nanofibers was investigated by scanning electron microscopy and transmission electron microscopy. The nanofibers exhibited a smooth surface and a narrow diameter distribution, with 60% of the nanofibers having diameters between 100 and 200 nm. Attenuated total reflectance-Fourier transform infrared spectra and X-ray photoelectron spectroscopy verified the existence of collagen molecules on the surface of nanofibers. Human coronary artery endothelial cells ( HCAECs) were seeded onto the blended nanofibers for viability, morphogenesis, attachment, and phenotypic studies. Five characteristic endothelial cell ( EC) markers, including four types of cell adhesion molecule and one EC-preferential gene ( von Willebrand factor), were studied by reverse transcription-polymerase chain reaction. Results showed that the collagen-blended polymer nanofibers could enhance the viability, spreading, and attachment of HCAECs and, moreover, preserve the EC phenotype. The blending electrospinning technique shows potential in refining the composition of polymer nanofibers by adding various ingredients ( e. g., growth factors) according to cell types to fabricate tissue-engineering scaffold, particularly blood vessel-engineering scaffold.