Characterization of a novel human type II epithelial keratin K1b, specifically expressed in eccrine sweat glands
Characterization of a novel human type II epithelial keratin K1b, specifically expressed in eccrine sweat glands
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DOI：
10.1111/j.0022-202x.2005.23860.x
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发表时间：
2005-09-01
影响因子：
6.5
通讯作者：
Schweizer, J
中科院分区：
文献类型：
--
作者：
Langbein, L;Rogers, MA;Schweizer, J
在这项研究中，我们发现，一种新的人类II型上皮角蛋白，K1b，是专门表达在外泌汗腺的管腔导管细胞。以这种管腔K1b的表达作为参考，我们已经使用了大量的上皮角蛋白的抗体，系统地研究其表达的分泌球和两层的汗管，分为intraglandular，真皮内，和intraepidermal（acropellingium）段，后者被进一步细分为汗管脊和上intraepidermal导管。我们表明，（i）每个外分泌汗腺组织隔室表达自己的角蛋白模式，（ii）外周和管腔导管层表现出连续的角蛋白表达，两者都代表自我更新的细胞层，（iii）皮内导管和汗管脊显示迄今未知的长度变化，（iv）在所有细胞层中，腔细胞层是最坚固的层，并且表达最高数量的角蛋白，这些角蛋白集中在细胞的顶侧以形成角质层。我们提供的证据表明，外周和腔层的细胞和细胞间的特性反映了适应不同的功能。
In this study, we show that a novel human type II epithelial keratin, K1b, is exclusively expressed in luminal duct cells of eccrine sweat glands. Taking this luminal K1b expression as a reference, we have used antibodies against a plethora of epithelial keratins to systematically investigate their expression in the secretory globule and the two-layered sweat duct, which was divided into the intraglandular, intradermal, and intraepidermal (acrosyringium) segments, the latter being further subdivided into the sweat duct ridge and upper intraepidermal duct. We show that (i) each of the eccrine sweat gland tissue compartments expresses their own keratin patterns, (ii) the peripheral and luminal duct layers exhibit a sequential keratin expression, with both representing self-renewing cell layers, (iii) the intradermal duct and the sweat duct ridge display hitherto unknown length variations, and (iv) out of all cell layers, the luminal cell layer is the most robust layer and expresses the highest number of keratins, these being concentrated at the apical side of the cells to form the cuticle. We provide evidence that the cellular and intercellular properties of the peripheral and the luminal layers reflect adaptations to different functions.