The potential role of human endogenous retrovirus K10 in the pathogenesis of rheumatoid arthritis: a preliminary study
The potential role of human endogenous retrovirus K10 in the pathogenesis of rheumatoid arthritis: a preliminary study
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DOI：
10.1136/ard.2004.031146
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发表时间：
2006-05-01
影响因子：
27.4
通讯作者：
Nelson, PN
中科院分区：
文献类型：
--
作者：
Ejtehadi, HD;Freimanis, GL;Nelson, PN
目的：目的：探讨人内源性逆转录病毒K10（HERV-K10）与自身免疫性风湿病的相关性。方法：采用多重逆转录聚合酶链反应（RT-PCR）技术，检测类风湿关节炎患者HERV-K10 mRNA的表达。40例类风湿性关节炎患者，17例骨关节炎患者，和27名健康个体，从外周血单个核细胞（PBMC）提取总RNA，并使用多重RT-PCR分析HERV-K10 gag mRNA表达水平。结果：以组氨酰tRNA合成酶（histidyl tRNA synthetase，HtRNAS）基因为管家基因，优化的多重RT-PCR方法显示，类风湿关节炎组织中HERV-K10 gag mRNA的表达水平明显高于骨关节炎组织结论：类风湿性关节炎患者HERV-K10 gag区mRNA表达水平高于骨关节炎患者和健康对照者。这可能有助于类风湿性关节炎的发病机制。
Objective: To examine whether human endogenous retrovirus K10 is associated with autoimmune rheumatic disease.Design: A novel multiplex reverse transcription polymerase chain reaction (RT-PCR) system was developed to investigate HERV-K10 mRNA expression in patients with rheumatoid arthritis.Methods: 40 patients with rheumatoid arthritis, 17 with osteoarthritis, and 27 healthy individuals were recruited and total RNA was extracted from peripheral blood mononuclear cells (PBMCs) and analysed using multiplex RT-PCR for the level of HERV-K10 gag mRNA expression. Southern blot and DNA sequencing confirmed the authenticity of the PCR products.Results: Using the histidyl tRNA synthetase (HtRNAS) gene as a housekeeping gene in the optimised multiplex RT-PCR, a significantly higher level of HERV-K10 gag mRNA expression was found in rheumatoid arthritis than in osteoarthritis ( p = 0.01) or in the healthy controls ( p = 0.02).Conclusion: There is enhanced mRNA expression of the HERV-K10 gag region in rheumatoid arthritis compared with osteoarthritis or healthy controls. This could contribute to the pathogenesis of rheumatoid arthritis.