Long term recordings with microelectrode arrays: Studies of transcription-dependent neuronal plasticity and axonal regeneration
Long term recordings with microelectrode arrays: Studies of transcription-dependent neuronal plasticity and axonal regeneration
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DOI：
10.1016/j.jphysparis.2005.12.005
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发表时间：
2006-03-01
影响因子：
--
通讯作者：
Bading, H
中科院分区：
文献类型：
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作者：
Hofmann, F;Bading, H
基板集成微电极阵列（MEA）提供了一种替代经典的电生理学方法，如膜片钳技术，用于记录神经元或心脏起源的细胞和组织的电活动。自30年前引入以来，这项技术已经能够以非侵入性的方式从多个地点重复同时记录。MEA技术可以应用于任何产电细胞或组织（即，中枢和外周神经元、心脏细胞和肌肉细胞），作为培养物或急性细胞或切片制备物。培养技术和多边环境协定的结合提供了在长达数月的时间内监测设计标本的活性的可能性。此外，记录制备物的分布区域的电活动产生关于空间效应的信息，这些信息可能用其他记录方法检测不到。发育、可塑性和再生是可以特别受益于长期监测神经元活动的应用的示例，因为它们涉及在延长的时间段内发育的过程。在这里，我们强调最近MEA的神经网络行为和轴突再生的信号调节的研究。我们说明了使用MEA研究长时程增强（LTP），并总结了MEA技术的优势，在传统的电生理方法的研究，旨在了解转录依赖的后期可塑性。(c)2005爱思唯尔有限公司保留所有权利。
Substrate integrated microelectrode arrays (MEAs) offer an alternative to classical electrophysiological methods like the patch clamp technique for recording the electrical activity from cells and tissue of neuronal or cardiac origin. Since its introduction 30 years ago, this technology has made possible the repeated simultaneous recording from multiple sites in a non-invasive manner. The MEA technology can be applied to any electrogenic cells or tissue (i.e., central and peripheral neurons, heart cells, and muscle cells), either as cultures or acute cell or slice preparations. The combination of culture techniques and MEAs offers the possibility to monitor the activity of a designed specimen over extended periods of time, up to several months. Furthermore, recording the electrical activity of distributed regions of a preparation yields information on spatial effects that might go undetected with other recording methods. Development, plasticity, and regeneration are examples of applications that could especially benefit from long term monitoring of neuronal activity, as they concern processes that develop over extended periods of time. Here we highlight recent MEA studies on signal regulation of neuronal network behavior and axonal regeneration. We illustrate the use of MEAs to study long term potentiation (LTP) and summarize the advantages of MEA technology over traditional electrophysiological methods for studies aimed at understanding the transcription-dependent late phase of plasticity. (c) 2005 Elsevier Ltd. All rights reserved.