PCR identification of Mycobacterium bovis BCG
PCR identification of Mycobacterium bovis BCG
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DOI：
10.1128/jcm.35.3.566-569.1997
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发表时间：
1997-03-01
影响因子：
9.4
通讯作者：
Frothingham, R
中科院分区：
文献类型：
--
作者：
Talbot, EA;Williams, DL;Frothingham, R
减毒卡介苗（BCG）疫苗株来源于牛分枝杆菌的强毒株。BCG很难与其他M. bovis和M.结核病综合征的常规方法。最近，一个被命名为RD1的基因组区域被发现存在于所有的毒性M。bovis和M.结核菌株测试，但从所有测试的BCG菌株中删除。利用这些信息，开发了一种多重PCR方法来检测RD1缺失。大量收集BCG和其他M。测试了来自不同宿主和地理来源的结核复合菌菌株。23株BCG中有23株缺失RD1。129例其他M中129例存在RD1。结核复合菌株。该多重PCR方法可作为快速、特异性鉴定卡介苗的工具。
The attenuated bacillus Calmette-Guerin (BCG) vaccine strain is derived from a virulent strain of Mycobacterium bovis. BCG is difficult to differentiate from other strains of M. bovis and other members of the M. tuberculosis complex by conventional methods. Recently, a genomic region designated RD1 was found to be present in all virulent M. bovis and M. tuberculosis strains tested but deleted from all BCG strains tested. With this information, a multiplex PCR method was developed to detect the RD1 deletion. A large collection of BCG and other M. tuberculosis complex strains from diverse host and geographic origins was tested. RD1 was deleted in 23 of 23 BCG strains. RD1 was present in 129 of 129 other M. tuberculosis complex strains. This multiplex PCR method can be used as a tool for the rapid and specific identification of BCG.