Development of SSR-based sorghum (Sorghum bicolor (L.) Moench) diversity research set of germplasm and its evaluation by morphological traits
Development of SSR-based sorghum (Sorghum bicolor (L.) Moench) diversity research set of germplasm and its evaluation by morphological traits
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DOI：
10.1007/s10722-008-9403-1
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发表时间：
2009-09-01
影响因子：
2
通讯作者：
Okuno, Kazutoshi
中科院分区：
文献类型：
--
作者：
Shehzad, Tariq;Okuizumi, Hisato;Okuno, Kazutoshi
植物遗传资源多样性的评价和利用是植物物种改良的重要内容。利用SSR标记构建了高粱多样性研究集（SDRS）。基于地理分布从包括亚洲（东亚、东南亚、南亚和西南亚）和非洲的3，500多份种质资源中选择了总共320份高粱资源，这些种质资源保存在日本NIAS Genebank中。从3个不同的高粱SSR连锁图谱中筛选出38个SSR标记，共产生146个等位基因，覆盖高粱的10条染色体。本研究中观察到的多态位点百分率（P）和基因多样性（He）平均值分别为82.8%和0.217。分析表明，与地理分化格局呈正相关。在SSR标记评价的基础上，选取107份高粱种质作为多样性研究集。SDRS群体与基础群体的遗传谱型差异不显著。相似地，没有观察到更大的变化的变异参数（骰子，%P，他）和几乎所有的SSR等位基因被保留在选定的高粱加入除了一个单一的等位基因在基因座Xtxp 287的损失。SDRS在高粱播种季节播种，重复两次。根据Genebank的高粱标准描述符，记录了26个重要形态性状的数据。方差分析表明，所有性状在所有种质间均存在极显著差异。形态农艺性状不能有效地根据地理来源进行聚类分析。
Assessment and utilization of diversity in plant genetic resources is vital for the improvement of plant species. A sorghum diversity research set (SDRS) was developed by using SSR markers. A total of 320 sorghum accessions were selected based on geographic distribution from more than 3,500 germplasm accessions comprising Asia (East, Southeast, South and Southwest Asia) and Africa, conserved at NIAS Genebank, Japan. We selected 38 simple sequence repeats (SSR) markers which generated 146 alleles, covering ten chromosomes of sorghum from a three different published SSR linkage map of sorghum. The average percentage of polymorphic loci (P) and gene diversity (He) observed in this study were 82.8 and 0.217 respectively. Analysis showed a positive correlation with geographic pattern of differentiation. Based on SSR assessment, 107 sorghum accessions were selected as diversity research set. There was no significant difference in pattern of genetic spectrum between SDRS and base population. Similarly no greater change was observed for variability parameters (Dice, %P, He) and almost all of the SSR alleles were retained in selected sorghum accessions except for the loss of a single allele at locus Xtxp287. SDRS was sown during sorghum sowing season in two replications. Data were recorded on 26 important morphological traits according to the standard sorghum descriptors at Genebank. Analysis of variance showed a highly significant difference among all accessions for all of the traits. Morpho-agronomic traits could not effectively classify the accessions according to geographic origin by using cluster analysis.