E3 ubiquitin ligase, RNF139, inhibits the progression of tongue cancer.
E3 ubiquitin ligase, RNF139, inhibits the progression of tongue cancer.
复制标题
E3泛素连接酶RNF139抑制舌癌的进展
DOI：
10.1186/s12885-017-3438-7
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发表时间：
2017-06-29
期刊：
影响因子：
3.8
通讯作者：
Shi C
中科院分区：
文献类型：
--
作者：
Wang L;Yin W;Shi C
背景舌癌仍然是世界范围内死亡的主要原因之一。最近，泛素系统已被确定为肿瘤的关键调节剂。为了寻找与口腔癌相关的E3泛素连接酶，我们筛选了人E3泛素连接酶文库，发现RING指蛋白139（RNF 139）对舌癌细胞的生物学行为具有调控作用。通过transwell法检测RNF 139基因敲除与否对SCC 9和SCC 25细胞侵袭能力的影响。免疫印迹法检测RNF 139在舌癌组织及癌旁组织中的表达水平。RNF 139对舌癌细胞致瘤性的影响进行了分析，通过异种移植模型对免疫缺陷Balb/c nude mice. ResultsRNF 139的过表达抑制舌癌细胞的生存能力，因为第2天。随着RNF 139的过表达，SCC 9和SCC 25细胞的殖民地形成能力也降低。RNF 139基因的敲除可显著增强SCC 9和SCC 25细胞的侵袭能力。此外，RNF 139的敲低也诱导AKT信号通路的激活。而舌癌组织中RNF 139的表达较低。在裸鼠中，敲低RNF 139促进了SCC 25 cells.ConclusionsOur data的致瘤性，确立了RNF 139在调节舌癌进展中的作用。
BackgroundTongue cancer is still one of the leading causes of mortality around the world. Recently, the ubiquitin system has been established as a critical modulator of tumors. In order to find the oral cancer related E3 ubiquitin ligases, we screened the human E3 ubiquitin ligase library and found that RING finger protein 139 (RNF139) regulated the biological behavior of tongue cancer cells.MethodsMTT assay was used to analyze the cell viability changes of tongue cancer SCC9 and SCC25 cells caused by RNF139. The invasion ability of SCC9 and SCC25 cells with or without the knockdown of RNF139 was evaluated through transwell assay. The immunoblotting was recruited to determine the expression level of RNF139 in human tongue cancer tissues and para-carcinoma tissues. The effect of RNF139 on tumorigenicity of tongue cancer cells was analyzed by xenograft model on immunodeficient Balb/c nude mice.ResultsOverexpression of RNF139 inhibits the viability of tongue cancer cells since day 2. The colony formation ability of SCC9 and SCC25 cells was also decreased with the overexpression of RNF139. Knockdown of RNF139 significantly promoted the invasion ability of SCC9 and SCC25 cells. Furthermore, knockdown of RNF139 also induced the activation of AKT signaling pathway. While human tongue cancer tissues had low expression of RNF139. In nude mice, knockdown of RNF139 promoted the tumorigenicity of the SCC25 cells.ConclusionsOur data establish a role for RNF139 in regulating the progression of tongue cancer.