A preliminary survey of extended-spectrum β-lactamases (ESBLs) in clinical isolates of Klebsiella pneumoniae and Escherichia coli in Japan
A preliminary survey of extended-spectrum β-lactamases (ESBLs) in clinical isolates of Klebsiella pneumoniae and Escherichia coli in Japan
复制标题
DOI：
10.1111/j.1574-6968.2000.tb08989.x
复制
发表时间：
2000-03-01
影响因子：
2.1
通讯作者：
Arakawa, Y
中科院分区：
文献类型：
--
作者：
Yagi, T;Kurokawa, H;Arakawa, Y
我们对1997年1月至1998年1月期间从196个独立医疗机构回收的16805株大肠埃希菌和9794株肺炎克雷伯菌临床分离株进行了超广谱β-内酰胺酶（ESBLs）调查。以羟亚氨基头孢菌素类抗生素的最小抑菌浓度（MIC）≥ 8 μ g ml（-1）为标准，并经双纸片法验证，共检出15株大肠埃希菌。大肠杆菌和34 K.产ESBLs肺炎分离株。用型特异性引物PCR法检测ESBLs基因型，除24株日本产ESBLs主要类型之一的Toho-1型外，还包括1株TEM型和24株SHV型ESBLs。SHV特异性PCR产物的核苷酸序列分析表明，SHV-12是SHV来源的ESBL的优势类型。此外，我们还鉴定了TEM-36和SHV-2。这是日本首次报道TEM和SHV衍生的ESBLs。(C)2000由Elsevier Science B. V.出版，保留所有权利。
We conducted a survey of extended-spectrum beta-lactamases (ESBLs) among 16 805 Escherichia coli and 9794 Klebsiella pneumoniae clinical isolates recovered from 196 separate medical institutions during the period January 1997 to January 1998. Using the criteria for minimal inhibitory concentrations (MICs) of oxyimino-cephalosporins of greater than or equal to 8 mu g ml(-1) and confirmation by double-disk test, we detected 15 E. coli and 34 K. pneumoniae isolates producing ESBLs. Genotypes of ESBLs determined by PCR with type-specific primers included one TEM-derived and 24 SHV-derived ESBLs, in addition to 24 Toho-1-type ESBLs, one of the major types of ESBLs reported in Japan. Nucleotide sequence analysis of SHV-specific PCR products revealed that SHV-12 was the dominant type of SHV-derived ESBL. In addition, we also identified TEM-36 and SHV-2. This is the first report characterizing TEM- and SHV-derived ESBLs in Japan. (C) 2000 Published by Elsevier Science B.V. All rights reserved.