Comparison of mammosphere formation from breast cancer cell lines and primary breast tumors
Comparison of mammosphere formation from breast cancer cell lines and primary breast tumors
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DOI：
10.3978/j.issn.2072-1439.2014.03.38
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发表时间：
2014-06-01
影响因子：
2.5
通讯作者：
Yang, Xiaoqin
中科院分区：
文献类型：
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作者：
Wang, Rong;Lv, Qing;Yang, Xiaoqin
背景：乳腺癌干细胞（BCSC）可以通过在非贴壁非分化条件下培养细胞来富集。然而，从原发性乳腺肿瘤中培养乳腺球成本高昂且难以控制。为了克服与使用原代人组织相关的问题，连续的乳腺癌细胞系已经从不同的sources.Methods：在这项研究中，管腔亚型乳腺癌细胞系MCF-7和基底亚型细胞系MDA-MB-231被选择。我们从两种细胞系和原发性乳腺肿瘤中探索BCSCs的最佳培养体系。比较两种乳腺癌细胞系和乳腺癌原发瘤的乳腺球形成率（MFE）、乳腺球中CD 44（+）/CD 24（-/low）ESA（+）Lin（-）细胞比例以及乳腺球的致瘤性。3种细胞均可形成乳腺球，其中MCF-7的MFE最高。培养3周后，MCF-7、MDA-MB-231细胞和原发性乳腺肿瘤的乳腺球中CD 44（+）/CD 24（-/低）ESA（+）Lin（-）细胞比例分别为95.0%+/-2.5%、82%+/- 22%和21.5%+/-1.0%。来自MCF-7、MDA-MB-231 mammospheres但不是原发性mammospheres.Conclusions的总共1，000个细胞是致瘤性的：本研究验证了使用乳腺癌细胞系作为模型来阐明BCSCs的性质。
Background: Breast cancer stem cells (BCSCs) can be enriched by culturing of cells in non-adherent non-differentiating conditions. However, culturing mammospheres from primary breast tumors are costly and difficult to control. In order to overcome problems associated with using primary human tissues, continuous breast cancer cell lines have been developed from various sources.Methods: In this study, a luminal subtype breast cancer cell line MCF-7 and a basal subtype cell line MDA-MB-231 were chosen. We explored the optimal culturing system for BCSCs from the two cell lines and primary breast tumors. Then, mammosphere formation efficiency (MFE), CD44(+)/CD24(-/low)ESA(+)Lin(-) cell proportion in mammospheres, and tumorigenecity of mammospheres generated from the two breast cancer cell lines and primary breast tumors were compared.Results: Enzymatic digestion of 60 mins and the addition of B27 to the culture medium were optimal for mammosphere culturing. Mammospheres could be formed in all the three cells, in which MCF-7 had the highest MFE. After 3 weeks culture, CD44(+)/CD24(-/low)ESA(+)Lin(-) cell proportion in mammospheres from MCF-7, MDA-MB-231 cells and primary breast tumors was 95.0%+/- 2.5%, 82%+/- 22% and 21.5%+/- 1.0%, respectively. A total of 1,000 cells from MCF-7, MDA-MB-231 mammospheres but not primary mammospheres were tumorigenic.Conclusions: This study validates the use of breast cancer cell lines as models to elucidate the nature of BCSCs.