The phosphatidylcholine/lysophosphatidylcholine ratio in human plasma is an indicator of the severity of rheumatoid arthritis:: Investigations by 31P NMR and MALDI-TOF MS
The phosphatidylcholine/lysophosphatidylcholine ratio in human plasma is an indicator of the severity of rheumatoid arthritis:: Investigations by 31P NMR and MALDI-TOF MS
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DOI：
10.1016/j.clinbiochem.2005.06.006
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发表时间：
2005-10-01
影响因子：
2.8
通讯作者：
Arnold, K
中科院分区：
文献类型：
--
作者：
Fuchs, B;Schiller, E;Arnold, K
目的：脂质第二信使，例如溶血磷脂酰胆碱（LPC）参与炎性疾病，例如类风湿性关节炎（RA）的发病机制。不幸的是，LPC在复杂的混合物中存在于体液中的分析仍然是具有挑战性的。设计和方法：基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）被应用于磷脂（PL）分析的关节液的有机提取物RA患者以及相应的血浆。这些数据进行了比较，通过高分辨率的P-31 NMR spectroscopy.Results获得的结果：滑液可能会被血浆替代，因为这两种体液的分析给出了非常相似的结果。对接受TNF-α抑制剂（阿达木单抗（修美乐））治疗的患者进行检查，以研究疾病活动性的临床显著减弱是否伴随着血浆PL组成的变化。它将被证明，特别是血浆中的PC/LPC比率代表一个可靠的测量炎症和增加后therapeutic.Conclusions：由于血浆样本是现成的，我们的方法可能是有用的，以得出结论之前，受影响的关节穿刺是必要的，PC/LPC比率检测血浆中可能作为一个指标，RA在早期阶段。(c)2005年加拿大临床化学家协会。All rights reserved.
Objectives: Lipid second messengers, e.g. lysophosphatidylcholines (LPC) are involved in the pathogenesis of inflammatory diseases, for instance, rheumatoid arthritis (RA). Unfortunately, the analysis of LPC in complex mixtures as present in body fluids is still challenging.Design and methods: Matrix-assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI-TOF MS) was applied for phospholipid (PL) analysis of organic extracts of synovial fluids from patients with RA as well as the corresponding plasma. These data were compared with results obtained by high resolution P-31 NMR spectroscopy.Results: Synovial fluids may be replaced by plasma since the analysis of both body fluids gives very similar results. Patients undergoing treatment with TNF-alpha inhibitors (ADALIMUMAB (HUMIRA (R))) were examined in order to investigate whether the clinically-significant attenuation of disease activity is accompanied by changes of the PL composition of plasma. It will be shown that especially the PC/LPC ratios of plasma represent a reliable measure of inflammation and increase upon therapy.Conclusions: Since plasma samples are readily available, our approach might be useful to draw conclusions before puncture of the affected joints is necessary and the PC/LPC ratio detected in plasma may serve as an indicator of RA in early stages. (c) 2005 The Canadian Society of Clinical Chemists. All rights reserved.