Antimicrobial activity of cyanidin-3-O-glucoside-lauric acid ester against Staphylococcus aureus and Escherichia coli
Antimicrobial activity of cyanidin-3-O-glucoside-lauric acid ester against Staphylococcus aureus and Escherichia coli
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DOI：
10.1016/j.foodchem.2022.132410
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发表时间：
2022-02-16
期刊：
影响因子：
8.8
通讯作者：
Zheng, Jie
中科院分区：
文献类型：
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作者：
Li, Lili;Zhou, Ping;Zheng, Jie
酶促脂肪酸酰化花色苷提高了花色苷的亲脂溶解性，提高了花色苷的应用潜力。然而，对产品功能特性的评价是其应用的前提。本研究旨在探讨酰化花色苷-矢车菊素-3-O-葡萄糖苷-月桂酸酯（C3 G-LA）的抑菌潜力及其抑菌机理，为其应用提供指导。C3 G-LA对金黄色葡萄球菌具有显著的抗菌活性[最小抑菌浓度（MIC）= 0.3125 mg/mL]，对大肠杆菌具有中等活性（MIC = 5 mg/mL）。C3 G-LA对S.金黄色葡萄球菌浓度为0.625 mg/mL。膜完整性降低（在S. aureus和E. C3 G-LA处理后，细胞内ATP浓度（分别降低96%和92%）、细胞内pH（分别降低11%和9%）以及细胞形态的改变均表明C3 G-LA处理后细胞膜功能紊乱。这些结果表明，C3 G-LA可以作为替代食品防腐剂对抗食源性病原体。
Enzymatic acylation of anthocyanin with fatty acid improves its lipophilic solubility and application potential. Nevertheless, evaluation of functional properties of product is premise for application. This study investigated the antimicrobial potential and the underlying mechanisms of an acylated anthocyanin, namely, cyanidin-3-O-glucoside-lauric acid ester (C3G-LA), to provide guidelines for its application. C3G-LA exhibited outstanding antibacterial activity against Staphylococcus aureus [minimum inhibitory concentration (MIC) = 0.3125 mg/mL] and modest activity against Escherichia coli (MIC = 5 mg/mL). Moreover, C3G-LA manifested bactericide ability against S. aureus at 0.625 mg/mL. Decreases in membrane integrity (by 96% and 92% at MIC in S. aureus and E. coli, respectively), intracellular ATP concentration (by 96% and 92%) and intracellular pH (by 11% and 9%) and changes in cellular morphology altogether indicated the dysfunction of cell membrane under C3G-LA treatment. These findings demonstrated that C3G-LA could be adopted as an alternative food preservative against foodborne pathogens.