Escherichia coli O157:H7 induces stronger plant immunity than Salmonella enterica Typhimurium SL1344.
Escherichia coli O157:H7 induces stronger plant immunity than Salmonella enterica Typhimurium SL1344.
复制标题
DOI：
10.1094/phyto-09-12-0230-fi
复制
发表时间：
2013-04
期刊：
影响因子：
3.2
通讯作者：
Melotto M
中科院分区：
文献类型：
--
作者：
Roy D;Panchal S;Rosa BA;Melotto M
食用被人类致病菌污染的新鲜农产品已导致各种疾病爆发，有时甚至是致命的。在这项研究中，我们评估了全致病细菌大肠杆菌O157：H7和鼠伤寒沙门氏菌SL1344在拟南芥和生菜中诱导的植物防御反应。与SL1344不同，O157：H7在入侵前和入侵后的侵染阶段都能诱导植物产生较强的免疫力。例如，O157：H7即使在高相对湿度(RH)下也会引发气孔关闭；这种环境条件通常会削弱植物在田间和实验室条件下对细菌的防御能力。相反，SL1344诱导了一种短暂的气孔免疫。我们还观察到，与SL1344相比，感染O157：H7的拟南芥叶片中PR1基因的表达显著增加。这些结果表明，植物可能比其他植物更有效地识别和响应某些人类病原体。此外，气孔免疫可以减少人类病原体通过叶表皮的渗透，导致植物质外体中的细菌滴度较低，这表明可以采取额外的控制措施来防止食物污染。了解植物反应如何减少细菌污染，对于预防暴发和提高食品供应的安全性至关重要。
Consumption of fresh produce contaminated with bacterial human pathogens has resulted in various, sometimes deadly, disease outbreaks. In this study, we assessed plant defense responses induced by the fully pathogenic bacteria Escherichia coli O157:H7 and Salmonella enterica serovar Typhimurium SL1344 in both Arabidopsis thaliana and lettuce (Lactuca sativa). Unlike SL1344, O157:H7 induced strong plant immunity at both pre-invasion and post-invasion steps of infection. For instance, O157:H7 triggered stomatal closure even under high relative humidity (RH); an environmental condition that generally weakens plant defenses against bacteria in the field and laboratory conditions. SL1344 instead induced a transient stomatal immunity. We also observed that PR1 gene expression was significantly higher in Arabidopsis leaves infected with O157:H7 as compared to SL1344. These results suggest that plants may recognize and respond to some human pathogens more effectively than others. Furthermore, stomatal immunity can diminish the penetration of human pathogens through the leaf epidermis resulting in low bacterial titers in the plant apoplast suggesting that additional control measures can be employed to prevent food contamination. The understanding of how plant responses can diminish bacterial contamination is paramount in preventing outbreaks and improving the safety of food supplies.