Discrimination between 5-hydroxy methylcytosine and 5-methylcytosine by a chemically designed peptide
Discrimination between 5-hydroxy methylcytosine and 5-methylcytosine by a chemically designed peptide
复制标题
通过化学设计的肽区分 5-羟甲基胞嘧啶和 5-甲基胞嘧啶
DOI：
10.1039/c1cc12131f
复制
发表时间：
2011
影响因子：
4.9
通讯作者：
Akimitsu Okamoto
中科院分区：
文献类型：
--
作者：
Akiko Nomura;Kaori Sugizaki;Hiroyuki Yanagisawa;Akimitsu Okamoto
一种人工磷酸肽识别DNA中甲基化和羟甲基化胞嘧啶之间的差异。用酪氨酸取代的Sp1锌指多肽能有效区分5-甲基胞嘧啶、5-羟甲基胞嘧啶(HMC)和未甲基化胞嘧啶。该多肽的DNA识别特性不同于其他检测HMC的化学物质。
An artificial phosphopeptide recognized the difference between methylated and hydroxymethylated cytosines in DNA. The Sp1 zinc finger peptide substituted by phosphotyrosine effectively discriminated between 5-methylcytosine, 5-hydroxymethylcytosine (hmC) and unmethylated cytosine. The DNA recognition properties of the peptide differ from those of other chemicals that detect hmC.