Duration of induced thermal tolerance and tissue-specific expression of hsp/hsc70 in the eastern oyster, Crassostrea virginica and the pacific oyster, Crassostrea gigas
Duration of induced thermal tolerance and tissue-specific expression of hsp/hsc70 in the eastern oyster, Crassostrea virginica and the pacific oyster, Crassostrea gigas
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DOI：
10.1016/j.aquaculture.2011.04.004
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发表时间：
2011-07
期刊：
影响因子：
4.5
通讯作者：
S. A. Jackson;K. Uhlinger;J. Clegg
中科院分区：
文献类型：
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作者：
S. A. Jackson;K. Uhlinger;J. Clegg
我们比较了太平洋牡蛎(Crassostrea Gias)和东方牡蛎(Crassostrea Virgiica)的耐热性和热休克反应，它们都是非常有价值的商业物种。前者对高温的抵抗力要强得多，在亚致死性热休克(SHS)后2天达到最大诱导热耐受(IT)，并持续至少3周，这可能是任何动物诱导热耐受持续时间的记录。在两种牡蛎中都检测到77和72kD两种组成异构体和一种可诱导的69kD蛋白，它们的数量和SHS后的时间进程因所检查的组织而异。鱼鳃(线虫)中这三种蛋白质的合成根据~(14)C-氨基酸掺入判断，在SHS后2天内，GIGAS降至对照水平。然而，Western blotting显示，这些应激蛋白在组织中的水平受到热休克C。GIGAS仍然高于对照组，这表明SHS后蛋白质降解减少。在SHS前后的鳃、外套膜、内收肌和消化腺中检测到了这三种亚型的不同类型。所描述的一般特征。内收肌、鳃和外套膜内也可见巨细胞组织。Virgiica，尽管也注意到了一些差异。我们讨论了在研究牡蛎中的这些应激蛋白时，使用商用抗体的困难和重要性。
We compared thermotolerance and the heat shock response of Pacific oysters (Crassostrea gigas) and Eastern oysters (Crassostrea virginica), both are very valuable commercial species. The former were much more resistant to high temperature, reaching maximum induced thermotolerance (IT) by 2 days after a sublethal heat shock (SHS) and lasting for at least 3 weeks, a possible record for the duration of IT in any animal. Two constitutive isoforms of 77 and 72kD, and an inducible 69kD protein were detected in both oysters, their amounts and time course after SHS varied, depending on the tissue examined. Synthesis of these three proteins in gills (ctenidia) ofC. gigaswas reduced to control levels within 2 days after SHS as judged by14C-amino acid incorporation. However, western blotting showed that the levels of these stress proteins in tissues from heat shockedC. gigasremained higher than those in controls, suggesting a reduction in protein degradation after SHS. Variable patterns of these three isoforms were detected in gills, mantle, adductor muscle and digestive gland before and after SHS. The general features described forC. gigastissues were also observed in adductor muscle, gill and mantle ofC. virginica, although some differences were also noted. We discuss the difficulties and importance associated with the use of commercially-available antibodies in the study of these stress proteins in oysters.