Metabolic profiling of cholesterol and sex steroid hormones to monitor urological diseases.
Metabolic profiling of cholesterol and sex steroid hormones to monitor urological diseases.
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DOI：
10.1530/erc-16-0285
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发表时间：
2016-10
影响因子：
3.9
通讯作者：
Kim J
中科院分区：
文献类型：
--
作者：
Moon JY;Choi MH;Kim J
胆固醇和性类固醇激素包括雄激素和雌激素在泌尿系统疾病如前列腺癌的发展和进展中起关键作用。这种疾病仍然是男性最常见的恶性肿瘤，也是各种癌症的主要死亡原因。试图了解胆固醇和类固醇代谢在泌尿系统疾病中的作用已经进行了多年，但尽管如此，我们的机制和翻译的理解仍然难以捉摸。为了进一步评估这个问题，我们对代谢组学产生了兴趣;这是一门致力于系统研究细胞，组织，毛发和生物液体中生物活性代谢物的学科。最近，我们提供的证据表明，胆固醇和性类固醇代谢物的定量测量可以成功地实现使用人和小鼠模型的头发。这篇简短的综述文章的总体目标是介绍目前用于定量生物标志物检测开发的代谢组学技术，并为理解触发病理状态的潜在机制提供新的见解。此外，本综述将特别强调如何制备生物标本（例如，毛发纤维），量化分子谱并评估其在各种泌尿系统疾病中的临床意义。
Cholesterol and sex steroid hormones including androgens and estrogens play a critical role in the development and progression of urological diseases such as prostate cancer. This disease remains the most commonly diagnosed malignant tumor in men and is the leading cause of death from different cancers. Attempts to understand the role of cholesterol and steroid metabolism in urological diseases have been ongoing for many years, but despite this, our mechanistic and translational understanding remains elusive. In order to further evaluate the problem, we have taken an interest in metabolomics; a discipline dedicated to the systematic study of biologically active metabolites in cells, tissues, hair and biofluids. Recently, we provided evidence that a quantitative measurement of cholesterol and sex steroid metabolites can be successfully achieved using hair of human and mouse models. The overall goal of this short review article is to introduce current metabolomic technologies for the quantitative biomarker assay development and also to provide new insight into understanding the underlying mechanisms that trigger the pathological condition. Furthermore, this review will place a particular emphasis on how to prepare biospecimens (e.g., hair fiber), quantify molecular profiles and assess their clinical significance in various urological diseases.