Exploring Serum Levels of Brain Derived Neurotrophic Factor and Nerve Growth Factor Across Glaucoma Stages.
Exploring Serum Levels of Brain Derived Neurotrophic Factor and Nerve Growth Factor Across Glaucoma Stages.
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在青光眼阶段探索脑衍生的神经营养因子和神经生长因子的血清水平。
DOI：
10.1371/journal.pone.0168565
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Manni G
中科院分区：
文献类型：
--
作者：
Oddone F;Roberti G;Micera A;Busanello A;Bonini S;Quaranta L;Agnifili L;Manni G
目的探讨不同疾病严重程度的原发性开角型青光眼患者血清脑源性神经营养因子（BDNF）和神经生长因子（NGF）水平与青光眼形态学和功能参数的关系。对45例不同阶段青光眼患者和15例年龄匹配的健康对照者进行了视野测试、光谱域光学相干断层扫描测量视网膜乳头周围神经纤维层厚度和采血酶联免疫吸附法检测神经营养因子。对生物仪器和生化数据进行了统计分析和相关性研究。青光眼患者血清BDNF水平显著低于健康对照组（261.2±75.0 pg/ml vs 313.6±79.6 pg/ml, p = 0.03）。亚组分析显示，早期青光眼患者血清BDNF水平（253.8±40.7 pg/ml, p = 0.019）和中度青光眼患者血清BDNF水平（231.3±54.3 pg/ml, p = 0.04）显著低于健康对照组，晚期青光眼患者血清BDNF水平（296.2±103.1 pg/ml, p = 0.06）无显著差异。青光眼患者血清NGF水平显著低于健康对照组（4.1±1 pg/mL vs 5.5±1.2 pg/mL, p = 0.01）。亚组分析显示，与健康对照组相比，早期青光眼（3.5±0.9 pg/mL, p = 0.0008）和中度青光眼（3.8±0.7 pg/mL, p<0.0001）血清NGF水平显著降低，但晚期青光眼（5.0±0.7 pg/mL, p = 0.32）无显著降低。青光眼组和对照组血清BDNF水平与年龄、视野平均偏差或视网膜神经纤维层厚度无关，而青光眼组血清NGF水平与视野平均偏差显著相关（r2 = 0.26, p = 0.004）。BDNF和NGF血清水平在青光眼早期和中度阶段降低，这表明这两个因素可以进一步研究作为青光眼早期检测的潜在循环生物标志物。
To investigate the serum levels of Brain Derived Neurotrophic Factor (BDNF) and Nerve Growth Factor (NGF) in patients affected by primary open angle glaucoma with a wide spectrum of disease severity compared to healthy controls and to explore their relationship with morphological and functional glaucoma parameters. 45 patients affected by glaucoma at different stages and 15 age-matched healthy control subjects underwent visual field testing, peripapillary retinal nerve fibre layer thickness measurement using Spectral Domain Optical Coherence Tomography and blood collection for both neurotrophins detection by Enzyme-Linked Immunosorbent Assay. Statistical analysis and association between biostrumental and biochemical data were investigated. Serum levels of BDNF in glaucoma patients were significantly lower than those measured in healthy controls (261.2±75.0 pg/ml vs 313.6±79.6 pg/ml, p = 0.03). Subgroups analysis showed that serum levels of BDNF were significantly lower in early (253.8±40.7 pg/ml, p = 0.019) and moderate glaucoma (231.3±54.3 pg/ml, p = 0.04) but not in advanced glaucoma (296.2±103.1 pg/ml, p = 0.06) compared to healthy controls. Serum levels of NGF in glaucoma patients were significantly lower than those measured in the healthy controls (4.1±1 pg/mL vs 5.5±1.2 pg/mL, p = 0.01). Subgroups analysis showed that serum levels of NGF were significantly lower in early (3.5±0.9 pg/mL, p = 0.0008) and moderate glaucoma (3.8±0.7 pg/ml, p<0.0001) but not in advanced glaucoma (5.0±0.7 pg/ml, p = 0.32) compared to healthy controls. BDNF serum levels were not related to age, visual field mean deviation or retinal nerve fibre layer thickness either in glaucoma or in controls while NGF levels were significantly related to visual field mean deviation in the glaucoma group (r2 = 0.26, p = 0.004). BDNF and NGF serum levels are reduced in the early and moderate glaucoma stages, suggesting the possibility that both factors could be further investigated as potential circulating biomarkers for the early detection of glaucoma.