Elevation of protooncogene messenger RNAs in estrogen-induced kidney tumors in the hamster.
Elevation of protooncogene messenger RNAs in estrogen-induced kidney tumors in the hamster.
复制标题
雌激素诱导的仓鼠肾肿瘤中原癌基因信使 RNA 的升高。
DOI：
10.1093/carcin/13.4.601
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发表时间：
1992
期刊：
影响因子：
4.7
通讯作者：
Stancel,GM
中科院分区：
文献类型：
--
作者：
Liehr,JG;Chiappetta,C;Roy,D;Stancel,GM
C-fos、c-jun和c-MyCare等细胞癌基因在雌激素诱导的正常靶组织(如啮齿动物子宫)生长之前表达。这些基因水平的一过性增加是由雌二醇引起的，随后是DNA复制。在这项研究中，我们检测了这三个癌基因在雌激素诱导的叙利亚仓鼠肾脏肿瘤中的表达，以了解激素致癌的机制。所有长期服用雌二醇7个月或9个月的叙利亚雄性仓鼠均可诱发肾脏肿瘤，而长期服用5个月的动物则未发现肿瘤。与年龄匹配的未处理对照肾脏相比，雌激素处理的9个月龄仓鼠的肾脏肿瘤组织中os、myandjun的基因表达水平分别增加了15、4和6倍。三种原癌基因在肿瘤周围肾组织中的表达也均增加，但在肿瘤和肾组织中的转录本比例没有一致的规律。经过7个月的雌激素治疗后，肾脏肿瘤中c-fos、c-jun和c-myc转录本的含量也升高，其水平与较老的肿瘤相当。在仓鼠肾肿瘤腹壁转移瘤中，os、myandjun基因的表达水平分别比对照水平高9倍、12倍和3倍。在用雌二醇治疗5个月的仓鼠肾脏中，原癌基因的表达略有增加。C-fos、c-myc和c-Junin雌激素治疗5个月后与对照组的比值分别为1.4、1.1和1.3。C-fos、c-jun和c-myc等细胞癌基因的过度表达可能在雌二醇诱导和生长金黄地鼠肾肿瘤中起一定作用。
Cellular oncogenes such as c-fos, c-junand c-mycare expressed prior to estrogen-induced growth of normal target tissues such as rodent uterus. Transient increases in the levels of these genes are induced by the administration of estradiol and are followed by DNA replication. In this study, we examined the expression of these three oncogenes in estradiolinduced kidney tumors in Syrian hamsters in order to understand mechanistic aspects of hormonal carcinogenesis. Kidney tumors were induced in all male Syrian hamsters treated chronically with estradiol for 7 or 9 months, whereas neoplasms were not detected in animals treated for 5 months. mRNA levels offos,mycandjunwere elevated 15-, 4- and 6-fold respectively in kidney tumors of estradiol-treated hamsters (9 months) compared with age-matched untreated control kidneys. The expression of all three protooncogenes was also increased in the kidney tissue surrounding tumors, though there was no consistent pattern in the ratios of transcripts in the tumor and kidney tissues. After 7 months of estrogen treatment, kidney tumors also contained elevated amounts of c-fos, c-junand c-myctranscripts at levels comparable with older tumors. In abdominal metastases of hamster kidney tumors, mRNA levels offos,mycandjunwere elevated 9-, 12- and 3-fold respectively over control levels. In kidneys of hamsters treated with estradiol for 5 months, in which tumors were not yet detected, the expression of protooncogenes was slightly increased. Ratios of c-fos, c-mycand c-junin estrogen-treated (5 months) over control tissue were 1.4, 1.1and 1.3 respectively. Overexpression of cellular oncogenes such as c-fos, c-junand c-mycmay have played a role in the induction and growth of kidney tumors by estradiol in hamsters.