Effects of the biocide methylisothiazolinone on Xenopus laevis wound healing and tail regeneration
Effects of the biocide methylisothiazolinone on Xenopus laevis wound healing and tail regeneration
复制标题
杀菌剂甲基异噻唑啉酮对非洲爪蟾伤口愈合和尾部再生的影响
DOI：
10.1016/j.aquatox.2016.10.016
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发表时间：
2016
影响因子：
4.5
通讯作者：
Tseng, Ai-Sun
中科院分区：
文献类型：
--
作者：
Delos Santos, Nicole;Azmat, Summer;Cuenca, Yesenia;Drenth, Jessica;Lauper, Julia;Tseng, Ai-Sun
南非爪蛙（Xenopus laevis）作为适合环境研究的模型有着悠久的历史。它的胚胎和透明蝌蚪对环境高度敏感，其发育过程被很好地描述。它也适用于分子研究。这些特性使其能够用于快速识别和理解暴露引起的缺陷。为了研究化学物质暴露对水生动物的影响，非洲爪蟾胚胎和蝌蚪被暴露于杀菌剂甲基异噻唑啉酮 (MIT) 中。截尾后暴露于麻省理工学院的青蛙蝌蚪失去了自然再生能力。这种再生抑制导致包括脊髓、肌肉和脊索在内的组织无法再生。这种麻省理工学院依赖性再生缺陷是由于未能闭合截肢伤口造成的。一项伤口愈合试验显示，未经治疗的胚胎会在受伤后一天内闭合伤口，而接受麻省理工学院治疗的动物则保持开放性伤口，但伤口尺寸并没有缩小，也没有导致死亡。同时将 MIT 与含有硫醇基团的化学物质（如谷胱甘肽和 N-乙酰半胱氨酸）一起暴露，可以恢复蝌蚪正常的伤口愈合和再生反应。这些结果共同表明，接触 MIT 会损害非洲爪蟾的发育性伤口修复和组织再生。因此，这项研究揭示了 MIT 活动的新方面，并证明非洲爪蟾是一个非常适合促进未来研究化学暴露对损伤反应影响的模型。
The South African clawed frog,Xenopus laevis, has a strong history as a suitable model for environmental studies. Its embryos and transparent tadpoles are highly sensitive to the environment and their developmental processes are well described. It is also amenable for molecular studies. These characteristics enable its use for rapid identification and understanding of exposure-induced defects. To investigate the consequences of chemical exposure on aquatic animals,Xenopus laevisembryos and tadpoles were exposed to the biocide, methylisothiazolinone (MIT). Frog tadpoles exposed to MIT following tail amputation lost their natural regenerative ability. This inhibition of regeneration led to a failure to regrow tissues including the spinal cord, muscle, and notochord. This MIT-dependent regenerative defect is due to a failure to close the amputation wound. A wound healing assay revealed that while untreated embryos close their wounds within one day after injury, MIT-treated animals maintained open wounds that did not reduce in size and caused lethality. Concomitant exposure of MIT with chemicals containing thiol groups such as glutathione and N-acetyl cysteine restored normal wound healing and regeneration responses in tadpoles. Together these results indicate that exposure to MIT impairs developmental wound repair and tissue regeneration inXenopus laevis. Thus, this study reveals new aspects of MIT activity and demonstrates thatXenopus laevisis a well-suited model for facilitating future research into chemical exposure effects on injury responses.