An intestinal bacterial metabolite of ginseng protopanaxadiol saponins has the ability to induce apoptosis in tumor cells
An intestinal bacterial metabolite of ginseng protopanaxadiol saponins has the ability to induce apoptosis in tumor cells
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DOI：
10.1006/bbrc.1998.8690
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发表时间：
1998-05-29
影响因子：
3.1
通讯作者：
Saiki, I
中科院分区：
文献类型：
--
作者：
Wakabayashi, C;Murakami, K;Saiki, I
我们以前的研究表明，口服人参原人参二醇组分的体内抗转移作用是由其代谢成分M1介导的，并且M1能抑制肿瘤细胞的生长、侵袭和迁移，但人参皂苷不能。在此，我们研究了M1对肿瘤细胞生长的抑制机制。M_1对小鼠黑色素瘤细胞B16-BL6的增殖抑制作用呈时间和剂量依赖性，并伴随着细胞形态的改变。此外，M_1在40 M时可诱导细胞在24小时内死亡，荧光显微镜下可见M_1进入胞浆并迅速到达细胞核(约15分钟)。Western印迹分析显示，M1以时间依赖的方式迅速上调p27(Kip1)的表达，而下调c-Myc和Cyclin D1的表达。因此，M1对凋亡相关蛋白的调控是诱导细胞凋亡的原因之一，这可能是M1体内抗转移活性的原因之一。(C)1998年学术出版社。
Our previous study demonstrated that the in vivo anti-metastatic effect induced by oral administration of ginseng protopanaxadiol saponins was mediated by their metabolic component M1, and that the growth, invasion and migration of tumor cells were inhibited by M1 but not by ginsenosides. Here we investigated the inhibitory mechanism of M1 on the growth of tumor cells. M1 inhibited the proliferation of B16-BL6 mouse melanoma cells in a time-and dose-dependent manner, with accompanying morphological changes at the concentration of 20 mu M. In addition, at 40 mu M M1 induced apoptotic cell death within 24 h. Fluorescence microscopy revealed that dansyl M1 entered the cytosol and quickly reached the nuclei (approximately 15 min). Western blot analysis revealed that M1 rapidly up-regulated the expression of p27(Kip1), but down-regulated the expression of c-Myc and cyclin D1 in a time-dependent manner. Thus, the regulation of apoptosis-related proteins by M1 is responsible for the induction of apoptotic cell death, and this probably leads to the anti-metastatic activity in vivo. (C) 1998 Academic Press.