An organic-inorganic hybrid scaffold for the culture of HepG2 cells in a bioreactor
An organic-inorganic hybrid scaffold for the culture of HepG2 cells in a bioreactor
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DOI：
10.1016/j.biomaterials.2004.07.012
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发表时间：
2005-05-01
期刊：
影响因子：
14
通讯作者：
Huh, N
中科院分区：
文献类型：
--
作者：
Kataoka, K;Nagao, Y;Huh, N
最近，人们对生物人工肝（BAL）作为患者的桥梁支持和实验目的的模块的潜在效用非常感兴趣。径向流生物反应器（RFB）是一种灌注床/支架型生物反应器，可以作为BAL进行高功能的三维培养。生物反应器的功能不仅取决于其机械结构，还取决于其中的支架。在目前的研究中，我们研究了一种新的多孔有机-无机-混合支架在RFB中的可能用途。该支架以四乙氧基硅烷（TEOS）和聚二甲基硅氧烷（PDMS）为原料，采用溶胶-凝胶法制备，以筛选过的蔗糖颗粒为多孔剂。在多孔的TEOS-PDMS混合支架中，人肝癌细胞（HepG2）比在聚乙烯醇支架中更有效地增殖和形成细胞团。当在PDMS-TEOS中培养时，HepG2细胞分泌的白蛋白量比在单层培养中分泌的白蛋白量高3倍。为了将BAL应用于药理学研究和未来的临床应用，有必要开发一种方法来繁殖保持高度特异性功能的肝细胞。目前的结果表明，PDMS-TEOS可能是开发这种功能培养方法的一个有前途的支架。(C) 2004 Elsevier Ltd.版权所有。
Much interest has recently been shown in the potential utility of bioartificial liver (BAL) as a bridge support for patients and as a module for experimental purposes. A radial-flow bioreactor (RFB), one of the perfused bed/scaffold-type bioreactors, enables a highly functional three-dimensional culture as BAL. The functional capacity of bioreactors depends not only on their mechanistic structures but also on scaffolds packed in them. In the present study, we examined the possible utility of a new porous organic-inorganic-hybrid scaffold in an RFB. The scaffold was made from tetraethoxysilane (TEOS) and polydimethylsiloxane (PDMS) by a sol-gel method using sieved sucrose particles as a porogen. In the porous TEOS-PDMS hybrid scaffold, human hepatocellular carcinoma cells (HepG2) proliferated actively and formed cell clusters more efficiently than they did in a polyvinyl-alcohol scaffold. When cultivated in PDMS-TEOS, HepG2 cells secreted a similar tothree-fold greater amount of albumin than that secreted in a monolayer culture. For potential application of BAL to pharmacological studies and future clinical use, it is essential to develop a method to propagate liver cells that maintain highly specific functions. The present results indicate that PDMS-TEOS may be a promising scaffold for developing such functional culture methods. (C) 2004 Elsevier Ltd. All rights reserved.