Shape analysis of the histone octamer in solution.
Shape analysis of the histone octamer in solution.
复制标题
溶液中组蛋白八聚体的形状分析。
DOI：
10.1126/science.3704649
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Bunick,GJ
中科院分区：
文献类型：
--
作者：
Uberbacher,EC;Harp,JM;Wilkinson-Singley,E;Bunick,GJ
组蛋白八聚体的构象取决于用于溶解它的特定盐。在2M氯化钠中，八聚体的大小和形状与结晶核心核小体的组蛋白组分相似。相比之下，在3.5M硫酸铵中，八聚体是细长的，类似于尺寸约为114 × 62 × 62埃的椭圆体。这些结果表明，在硫酸铵中结晶的组蛋白八聚体的3.3埃电子密度图中看到的伸长构象是由于所使用的特定盐条件。
The conformation of the histone octamer is shown to depend upon the specific salt used to solubilize it. In 2Msodium chloride the octamer is similar in size and shape to the histone component of crystallized core nucleosomes. In contrast, in 3.5Mammonium sulfate the octamer is elongated, resembling an ellipsoid with approximate dimensions of 114 by 62 by 62 angstroms. These results indicate that the elongated conformation seen in the 3.3 angstroms electron density map of the histone octamer crystallized in ammonium sulfate is due to the particular salt conditions used.