The composition of carcass volatile profiles in relation to storage time and climate conditions
The composition of carcass volatile profiles in relation to storage time and climate conditions
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DOI：
10.1016/j.forsciint.2012.08.001
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发表时间：
2012-11-30
影响因子：
2.2
通讯作者：
Ruther, Joachim
中科院分区：
文献类型：
--
作者：
Kasper, Julia;Mumm, Roland;Ruther, Joachim
生物体死后会被各种酶和微生物分解。腐烂通常伴随着过多的挥发性有机化合物的排放，这些化合物造成尸体的难闻气味，从而吸引食尸昆虫。与尸体相关的气味特征的组成强烈取决于大量营养素的组成，如脂肪、碳水化合物，特别是蛋白质，以及影响定居在尸体上的微生物群落的氧气的存在。温度和湿度等非生物因素对尸体相关挥发性排放的影响尚不清楚，尽管已知这些参数对微生物的生长有很大影响。在本研究中，我们调查了从死小鼠中释放的挥发性物质的连续性，在温暖/炎热（wh，22摄氏度/80- 90%RH）或寒冷/干燥（cd，12摄氏度/40- 60%RH）的气候条件下储存1天，10天和30天。我们确定了51个典型的胴体挥发性物质的气相色谱-质谱联用，并通过多元统计方法分析的挥发性概况，以找到化合物表征的不同阶段。死小鼠在湿条件下储存释放挥发物更快，在更高的数额，并在一个更大的多样性比那些储存在cd条件。在cd条件下释放的含硫化学物质相对较低，这是最引人注目的。结果进行了讨论，其可能的适用性在法医学和昆虫生态学研究。(C)2012爱思唯尔爱尔兰有限公司保留所有权利。
After death organisms are decomposed by a variety of enzymes and microorganisms. The decay is typically accompanied by the emission of a plethora of volatile organic compounds responsible for the unpleasant odour of a carcass and thus, for the attraction of necrophagous insects. The composition of carcass-related odour profiles strongly depends on the composition of macro-nutrients like fat, carbohydrates, and particularly protein, as well as on the presence of oxygen which influences the community of microorganisms colonising the corpse. The impact of abiotic factors like temperature and humidity on carcass-related volatile emission is less well understood although these parameters are known to have a strong impact on the growth of microorganisms. In the present study we investigated the volatile succession released from dead mice stored for one, ten and 30 days under warm/hot (wh, 22 degrees C/80-90% RH) or cold/dry (cd, 12 degrees C/40-60% RH) climate conditions. We identified 51 typical carcass volatiles by coupled gas chromatography-mass spectrometry and analysed the volatile profiles by multivariate statistical methods to find compounds characterising the different stages. Dead mice stored under wh conditions released volatiles much faster, in higher amounts, and in a greater diversity than those stored under cd conditions. The relatively low amount of sulphur chemicals released under cd conditions were most striking. The results are discussed with respect to their possible applicability in forensic science and insect ecology studies. (C) 2012 Elsevier Ireland Ltd. All rights reserved.