Downregulation of connexin 45 gene products during mouse heart development.
Downregulation of connexin 45 gene products during mouse heart development.
复制标题
小鼠心脏发育过程中连接蛋白 45 基因产物的下调。
作者：
S. Alcoléa;M. Théveniau;T. Jarry‐guichard;I. Marics;Elena Tzouanacou;J. Chauvin;J. Briand;A. Moorman;W. Lamers;D. Gros
心脏的电活动在窦房结中产生，然后传播到心房和心室组织。耦合肌细胞的差距连接通道负责该传播过程。差距连接通道是连接蛋白（Cx）家族的跨膜蛋白的十二聚体。该家族的三个成员已被证明在心肌细胞中合成：Cx40、Cx43和Cx45。此外，它们中的每一种都已被证明形成具有独特和特定电生理特性的通道。理解传导现象需要详细了解这些Cx在心脏中的时空表达模式。Cx40和Cx43的表达模式已在成人心脏及其发育过程中进行了描述。在这里，我们报告的Cx45基因产物在小鼠心脏的表达，从第一次收缩阶段（8.5天postcobiliary [dpc]）到成年阶段。通过逆转录-聚合酶链反应实验证明Cx45基因转录本在研究的所有阶段都存在于心脏中。在8.5和10.5 dpc之间，通过原位杂交显示在所有心脏隔室（包括流入道和流出道以及房室管）中以低量表达，然后从11到12 dpc开始下调。在随后的胎儿阶段，在心室中检测到微弱的转录本，在流出道中表达最明显。通过免疫荧光显微镜证实，Cx45蛋白在年轻胚胎心脏（8.5至9.5 dpc）的心肌细胞中表达。然而，超过10.5 dpc的蛋白质不再检测与此技术在胚胎，胎儿，或新生儿的工作心肌，虽然它可以通过免疫印迹显示，该蛋白质仍在新生儿心脏合成。在成人心脏的大部分，Cx45是不可检测的。然而，在室间隔的前部区域可以清楚地看到，在心室游离壁中分散的一些小病灶中可以看到痕量。Cx45基因是迄今为止发现的第一个在心脏第一次收缩时被激活的Cx基因。因此，在此阶段发生的缓慢蠕动收缩期间，肌细胞的协调似乎是由Cx45通道控制的。
The electrical activity in heart is generated in the sinoatrial node and then propagates to the atrial and ventricular tissues. The gap junction channels that couple the myocytes are responsible for this propagation process. The gap junction channels are dodecamers of transmembrane proteins of the connexin (Cx) family. Three members of this family have been demonstrated to be synthesized in the cardiomyocytes: Cx40, Cx43, and Cx45. In addition, each of them has been shown to form channels with unique and specific electrophysiological properties. Understanding the conduction phenomenon requires detailed knowledge of the spatiotemporal expression pattern of these Cxs in heart. The expression patterns of Cx40 and Cx43 have been previously described in the adult heart and during its development. Here we report the expression of Cx45 gene products in mouse heart from the stage of the first contractions (8.5 days postcoitum [dpc]) to the adult stage. The Cx45 gene transcript was demonstrated by reverse transcriptase-polymerase chain reaction experiments to be present in heart at all stages investigated. Between 8.5 and 10.5 dpc it was shown by in situ hybridization to be expressed in low amounts in all cardiac compartments (including the inflow and outflow tracts and the atrioventricular canal) and then to be downregulated from 11 to 12 dpc onward. At subsequent fetal stages, the transcript was weakly detected in the ventricles, with the most distinct expression in the outflow tract. Cx45 protein was demonstrated by immunofluorescence microscopy to be expressed in the myocytes of young embryonic hearts (8.5 to 9.5 dpc). However, beyond 10.5 dpc the protein was no longer detected with this technique in the embryonic, fetal, or neonatal working myocardium, although it could be shown by immunoblotting that the protein was still synthesized in neonatal heart. In the major part of adult heart, Cx45 was undetectable. It was, however, clearly seen in the anterior regions of the interventricular septum and in trace amounts in some small foci dispersed in the ventricular free walls. Cx45 gene is the first Cx gene so far demonstrated to be activated in heart at the stage of the first contractions. The coordination of myocytes during the slow peristaltic contractions that occur at this stage would thus appear to be controlled by the Cx45 channels.
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影响因子：
3.2
作者：
BERRYMAN, MA;RODEWALD, RD
通讯作者：
RODEWALD, RD
间隙连接蛋白 connexin45 的表征。
DOI：
10.1007/bf00232672
发表时间：
1994
期刊：
The Journal of membrane biology
影响因子：
--
作者：
Laing,JG;Westphale,EM;Engelmann,GL;Beyer,EC
通讯作者：
Beyer,EC
影响因子：
20.1
作者：
DARROW, BJ;LAING, JG;BEYER, EC
通讯作者：
BEYER, EC
DOI：
10.1016/s0021-9258(18)77321-3
发表时间：
1990-08
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
E. Beyer
通讯作者：
E. Beyer
影响因子：
20.1
作者：
KANTER, HL;LAING, JG;SAFFITZ, JE
通讯作者：
SAFFITZ, JE