Rice Expression Atlas In Reproductive Development
Rice Expression Atlas In Reproductive Development
复制标题
DOI：
10.1093/pcp/pcq165
复制
发表时间：
2010-12-01
影响因子：
4.9
通讯作者：
Kurata, Nori
中科院分区：
文献类型：
--
作者：
Fujita, Masahiro;Horiuchi, Youko;Kurata, Nori
分析了从原基发育开始，经过授粉/受精到合子形成的整个水稻生殖过程中的基因表达。我们用57，381个探针组分析了包括小孢子发生早期在内的水稻生殖发育的25个阶段/器官，并在每个阶段鉴定了约26，000个表达探针组。25个生殖阶段/器官的精细解剖结合详细的微阵列分析揭示了基因表达的戏剧性，协调和微调的变化。拟南芥和玉米花粉成熟过程中表达基因的减少与此相似。几乎相同数量的从头预测基因和克隆基因的出现或消失与发育阶段的进展协调。鉴定了大量器官/阶段特异性基因;特别是2，593个用于发育花药的探针组，包括932个对应于从头预测基因的探针组。细胞周期相关基因的分析表明，几个细胞周期蛋白依赖性激酶（CDKs），细胞周期蛋白和SCF E3泛素连接酶复合物的成分在生殖器官中特异性表达。细胞壁生物合成或降解蛋白基因和转录因子基因在生殖阶段特异表达。与其他植物生殖相关基因同源的水稻基因的表达谱与预测的功能既有一致的，也有不一致的。水稻生殖表达图谱可能是最广泛和最全面的数据集，对于揭示许多尚未彻底分析的植物生殖过程中特定基因的功能是不可或缺的。
Gene expression throughout the reproductive process in rice (Oryza sativa) beginning with primordia development through pollination/fertilization to zygote formation was analyzed. We analyzed 25 stages/organs of rice reproductive development including early microsporogenesis stages with 57,381 probe sets, and identified around 26,000 expressed probe sets in each stage. Fine dissection of 25 reproductive stages/organs combined with detailed microarray profiling revealed dramatic, coordinated and finely tuned changes in gene expression. A decrease in expressed genes in the pollen maturation process was observed in a similar way with Arabidopsis and maize. An almost equal number of ab initio predicted genes and cloned genes which appeared or disappeared coordinated with developmental stage progression. A large number of organ-/stage-specific genes were identified; notably 2,593 probe sets for developing anther, including 932 probe sets corresponding to ab initio predicted genes. Analysis of cell cycle-related genes revealed that several cyclin-dependent kinases (CDKs), cyclins and components of SCF E3 ubiquitin ligase complexes were expressed specifically in reproductive organs. Cell wall biosynthesis or degradation protein genes and transcription factor genes expressed specifically in reproductive stages were also newly identified. Rice genes homologous to reproduction-related genes in other plants showed expression profiles both consistent and inconsistent with their predicted functions. The rice reproductive expression atlas is likely to be the most extensive and most comprehensive data set available, indispensable for unraveling functions of many specific genes in plant reproductive processes that have not yet been thoroughly analyzed.