Nanos is the localized posterior determinant in Drosophila
Nanos is the localized posterior determinant in Drosophila
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DOI：
10.1016/0092-8674(91)90110-k
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发表时间：
1991-08
期刊：
影响因子：
64.5
通讯作者：
Charlotte I. Wang;R. Lehmann
中科院分区：
文献类型：
--
作者：
Charlotte I. Wang;R. Lehmann
果蝇胚胎的节段模式是由位于卵细胞前极和后极的两个母体因素建立的。在这里，我们提供了分子证据，证明局部后因子是 nanos (nos) 基因的 RNA。 nos RNA 定位于早期胚胎的后极，nos 蛋白在一定距离内发挥作用，指导腹部的形成。合成的 nos RNA 具有与后极血浆相同的生物活性。注射 nos RNA 可挽救所有九个已知后群基因突变的雌性胚胎的分割缺陷。将 nos RNA 注射到前部能够直接形成异位后部结构。我们的结果表明，nos RNA 的局部来源足以指定腹部分割，并意味着 nos 基因产物的定位、稳定或分布需要其他后组基因。
Segmental pattern in the Drosophila embryo is established by two maternal factors localized to the anterior and posterior poles of the egg cell. Here we provide molecular evidence that the localized posterior factor is the RNA of the nanos (nos) gene. nos RNA is localized to the posterior pole of early embryos, and nos protein acts at a distance to direct abdomen formation. Synthetic nos RNA has biological activity identical to that of the posterior pole plasm. Injection of nos RNA rescues the segmentation defect of embryos derived from females mutant for all nine known posterior group genes. Injection of nos RNA into the anterior is able to direct formation of ectopic posterior structures. Our results demonstrate that a localized source of nos RNA is sufficient to specify abdominal segmentation and imply that other posterior group genes are required for localization, stabilization, or distribution of the nos gene product.