Respiratory burst of Biomphalaria glabrata hemocytes:: Schistosoma mansoni-resistant snails produce more extracellular H2O2 than susceptible snails
Respiratory burst of Biomphalaria glabrata hemocytes:: Schistosoma mansoni-resistant snails produce more extracellular H2O2 than susceptible snails
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DOI：
10.1645/ge-415r
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发表时间：
2005-04-01
影响因子：
1.3
通讯作者：
Bayne, CJ
中科院分区：
文献类型：
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作者：
Bender, RC;Broderick, EJ;Bayne, CJ
来自腹足动物光滑双脐螺（Biomphalaria glabrata）的血细胞产生活性氧与它们杀死吸虫寄生虫曼氏血吸虫（Schistosoma mansoni）的能力有关。对2个实验室品系的钉螺，1个品系（13-16-R1）对PR_1株抗性，1个品系（MO）敏感。mansoni，我们比较了血细胞产生的细胞外过氧化氢时，刺激蛋白激酶C激动剂佛波醇肉豆蔻酸酯乙酸酯（PMA）。就呼吸爆发的开始、峰值产生和终止而言，两种菌株中PMA诱导反应的时间过程相似。然而，不同菌株的反应程度不同，因为来自抗性蜗牛的血细胞产生更多的过氧化氢。这些发现表明，产生过氧化氢的能力可能是决定敏感性或耐药性的关键。mansoni
The production of reactive oxygen species by hemocytes from the gastropod Biomphalaria glabrata has been linked to their ability to kill the trematode parasite Schistosoma mansoni. For 2 laboratory strains of the snail, 1 resistant (13-16-R1) and 1 susceptible (MO) to the PR1 strain of S. mansoni, we compared hemocyte production of extracellular hydrogen peroxide when stimulated with the protein kinase C agonist phorbol myristate acetate (PMA). The time course of the PMA-induced response is similar in both strains with respect to onset, peak production, and termination of the respiratory burst. However, the magnitude of the response differs between strains, in that hemocytes from resistant snails generate significantly more hydrogen peroxide. These findings suggest that the capacity to produce hydrogen peroxide could be critical in determining susceptibility or resistance to S. mansoni.