How to realize the linear scale-up process for rapid purification using high-performance counter-current chromatography.
How to realize the linear scale-up process for rapid purification using high-performance counter-current chromatography.
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DOI：
10.1016/j.chroma.2008.04.049
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发表时间：
2008-06
期刊：
影响因子：
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通讯作者：
Yuan Yuan-Yuan;Biqin Wang;Li-juan Chen;H. Luo;D. Fisher;I. Sutherland;Yuquan Wei
中科院分区：
文献类型：
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作者：
Yuan Yuan-Yuan;Biqin Wang;Li-juan Chen;H. Luo;D. Fisher;I. Sutherland;Yuquan Wei
本研究以卷柏中六种成分的分离为例，演示了如何在正相和反相高效逆流色谱中实现快速和可预测的线性放大过程。在对Mini-DE分析型离心机的溶剂系统、样品浓度、上样量、转速和流速进行系统优化后，将优化后的参数直接转移到制备型Midi-DE离心机上，其纯度、分离度和洗脱时间几乎相同，但通量提高了50倍。获得穗花杉双黄酮（446.7mg，97.8%）、粗壮黄酮（21.6mg，89.4%）、白果黄酮（80.7mg，92.7%）、扁柏黄酮（15.1mg，85.5%）、异隐黄素（34.8mg，89.6%）和芹菜素-二葡糖苷（46.3mg，96.4%），如通过HPLC分析的，量和纯度在括号中示出。因此，该方法提供了一种获得足够量的目标化合物的有效且快速的方法，在线性放大后具有显著增加的通量。
This study used the isolation of six constituents from Selaginella tamariscina as an example to demonstrate how to achieve rapid and predictable linear scale-up processes in both normal- and reversed-phase high-performance counter-current chromatography. After systematic optimization of solvent systems, sample concentration, sample loading volume, rotation speed and flow rate on the analytical Mini-DE centrifuge, the optimized parameters obtained were directly transferred to the preparative Midi-DE centrifuge, with nearly the same purities, resolutions and elution times but with 50 times the throughput. Amentoflavone (446.7mg, 97.8%), robustaflavone (21.6mg, 89.4%), bilobetin (80.7mg, 92.7%), hinokiflavone (15.1mg, 85.5%), isocryptomerin (34.8mg, 89.6%) and an apigenin-diglucoside (46.3mg, 96.4%) were obtained with amounts and purities shown in parentheses as analysed by HPLC. The process, therefore, offers an efficient and rapid method of obtaining sufficient quantities of target compounds with significantly increased throughput after a linear scale-up.