Identification and redistribution of lamins during nuclear differentiation in mouse spermatogenesis.
Identification and redistribution of lamins during nuclear differentiation in mouse spermatogenesis.
复制标题
小鼠精子发生核分化过程中核纤层蛋白的识别和重新分布。
DOI：
10.1016/0012-1606(86)90228-9
复制
发表时间：
1986
影响因子：
2.7
通讯作者：
Bechtol,KB
中科院分区：
文献类型：
--
作者：
Maul,GG;French,BT;Bechtol,KB
染色质可以通过核膜层蛋白A、B和C的相互作用附着到核膜上。这种假设要求这些蛋白质存在于所有染色质附着于核膜的细胞中。我们研究了核纤层蛋白在小鼠精子发生过程中的分布，这表现出极端的核膜结构变化。通过免疫组织化学技术，使用人类自身抗体和单克隆抗体对这些分子，我们发现，层粘连蛋白坚持通过精子发生的所有阶段，虽然在高度可变的金额。它们也存在于减数分裂前期（粗线期），此时染色体仅局部附着于核膜，类似于体细胞的早期前期。早期精子细胞核膜的重组伴随着顶体窝新核纤层蛋白的出现。在附睾精子中，不同核纤层蛋白在细胞核上的分布差异很大，表明其具有特殊的结构功能。整个精子发生过程中核纤层蛋白的存在支持了这样一个概念，即它们是核膜结构的一般特征，即使在超微结构上不能识别核纤层。
Chromatin may be attached to the nuclear envelope through interaction of the nuclear membrane lamins A, B, and C. Such a hypothesis requires that these proteins are present in all cells with chromatin attachment to the nuclear envelope. We have investigated the distribution of the lamins during spermatogenesis in mouse, which exhibits extremes in nuclear envelope structural changes. By immunohistochemical techniques using human auto-antibodies and monoclonal antibodies against these molecules, we found that the lamins persist through all stages of spermatogenesis, though in highly variable amounts. They are also present during meiotic prophase (pachytene) when chromosomes are only locally attached to the nuclear envelope, analogous to the early prophase of somatic cells. Restructuring of the early spermatid nuclear envelope is accompanied by the appearance of a new lamin at the acrosomal fossa. In the epididymal spermatozoon the distribution of different lamins varies markedly over the nucleus suggesting special structural functions. The presence of lamins throughout spermatogenesis supports the concept that they are a general feature of the nuclear envelope structure, even where a lamina is not recognizable ultrastructurally.