Changes in the bacterial flora of the neoterminal ileum after ileocolonic resection for Crohn's disease
Changes in the bacterial flora of the neoterminal ileum after ileocolonic resection for Crohn's disease
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DOI：
10.1111/j.1572-0241.2002.05613.x
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发表时间：
2002-04-01
影响因子：
9.8
通讯作者：
Colombel, JF
中科院分区：
文献类型：
--
作者：
Neut, C;Bulois, P;Colombel, JF
目的：细菌制剂与回结肠切除术后克罗恩病的早期复发有关。我们的研究的目的是确定和量化与患者和对照组的回肠粘膜相关的细菌，并与特定的细菌recurrence.METHODS：回肠的主要细菌菌群进行了枚举和鉴定，需氧和厌氧，在活检时获得的手术或内窥镜检查61例克罗恩病和10回结肠切除术对照。这61份标本包括13份取自切除标本的回肠活检标本。回肠造口术后7例，回肠结肠切除术后41例。结果：回肠结肠切除术诱导了患者和对照组新末端回肠中细菌计数和种类的显著增加，而回肠造口术活组织检查中未观察到。患者和对照组之间的比较显示，更多的大肠杆菌和肠球菌在克罗恩病和双歧杆菌和瘤胃球菌在对照组。早期复发与高E.结论：回结肠切除术后，新生末端回肠定植增加。我们的数据表明，特定细菌如大肠杆菌的数量增加。大肠杆菌、肠球菌、类杆菌和梭菌可能是克罗恩病术后复发的重要因素。
OBJECTIVE: Bacterial agents have been implicated in the early recurrence of Crohn's disease after ileocolectomy. The aim of our study was to identify and quantify bacteria associated with the ileal mucosa in patients and controls and to correlate specific bacteria with recurrence.METHODS: The predominant bacterial microflora of the ileum were enumerated and identified, aerobically and anaerobically, in biopsies obtained at the time of surgery or by endoscopy from 61 patients with Crohn's disease and 10 ileocolectomy controls. The 61 specimens were comprised of 13 ileal biopsies taken from resection specimens. seven taken after ileostomy, and 41 taken after ileocolectomy.RESULTS: Ileocolectomy induced a significant increase in bacterial counts and variety in the neoterminal ileum in both patients and controls that was not observed in ileostomy biopsies. Comparison between patients and controls revealed greater numbers of Escherichia coli and enterococci in Crohn's disease and of bifidobacteria and ruminococci in controls. Early recurrence was associated with high counts of E. coli and bacteroides and the frequent isolation of fusobacteria.CONCLUSION: After ileocolectomy, colonization of the neoterminal ileum is increased. Our data suggest that increases in the populations of specific bacteria such as E. coli, enterococci, bacteroides, and fusobacteria may be important in postoperative recurrence of Crohn's disease.