SPECIFIC ASSOCIATION OF HUMAN TELOMERASE ACTIVITY WITH IMMORTAL CELLS AND CANCER
SPECIFIC ASSOCIATION OF HUMAN TELOMERASE ACTIVITY WITH IMMORTAL CELLS AND CANCER
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DOI：
10.1126/science.7605428
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发表时间：
1994-12-23
期刊：
影响因子：
56.9
通讯作者：
SHAY, JW
中科院分区：
文献类型：
--
作者：
KIM, NW;PIATYSZEK, MA;SHAY, JW
端粒酶在染色体末端合成DNA可能是人类细胞无限增殖所必需的。开发了一种用于测量端粒酶活性的高灵敏度测定方法。在代表18种不同人体组织的培养细胞中，100个永生群体中有98个端粒酶阳性，22个死亡群体中没有一个端粒酶阳性。同样，代表12种人类肿瘤类型的101个活检中有90个呈阳性，50个正常体细胞组织中没有一个呈阳性。正常卵巢和睾丸呈阳性，而良性肿瘤如肌瘤呈阴性。因此，端粒酶似乎在正常的人体组织中受到严格抑制，但在癌症中被重新激活，而癌症可能需要永生细胞来维持肿瘤的生长。
Synthesis of DNA at chromosome ends by telomerase may be necessary for indefinite proliferation of human cells. A highly sensitive assay for measuring telomerase activity was developed. In cultured cells representing 18 different human tissues, 98 of 100 immortal and none of 22 mortal populations were positive for telomerase. Similarly, 90 of 101 biopsies representing 12 human tumor types and none of 50 normal somatic tissues were positive. Normal ovaries and testes were positive, but benign tumors such as fibroids were negative. Thus, telomerase appears to be stringently repressed in normal human somatic tissues but reactivated in cancer, where immortal cells are likely required to maintain tumor growth.