Neolacto-series gangliosides induce granulocytic differentiation of human promyelocytic leukemia cell line HL-60.
Neolacto-series gangliosides induce granulocytic differentiation of human promyelocytic leukemia cell line HL-60.
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新乳系列神经节苷脂诱导人早幼粒细胞白血病细胞系 HL-60 的粒细胞分化。
DOI：
10.1016/s0021-9258(18)68515-1
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Masaki Saito
中科院分区：
文献类型：
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作者：
H. Nojiri;S. Kitagawa;Mitsuru Nakamura;K. Kirito;Y. Enomoto;Masaki Saito
具有线性聚-N-乙酰基-乳糖胺寡糖结构的新乳糖系列神经节苷脂已被证明在由二甲亚砜或视黄酸诱导的人早幼粒细胞白血病细胞系HL-60细胞的粒细胞分化期间特征性地增加（Nojiri，H.，Takaku，F.，Tetsuka，T.，Motoyoshi，K.，Miura，Y.，Saito，M.（1984）Blood 64，534-541）。用成熟粒细胞制备的新乳系神经节苷脂与HL-60细胞共培养，观察细胞形态、表面膜抗原、非特异性酯酶活性、吞噬和呼吸爆发活性的变化，发现HL-60细胞可分化为成熟粒细胞。细胞的分化依赖于神经节苷脂的浓度，并伴有细胞生长抑制。这些结果表明，特定的神经节苷脂分子在细胞分化的调节中起着重要作用，细胞表面膜上的新乳糖系列神经节苷脂的出现不仅触发了HL-60细胞的分化，而且决定了分化的方向。
Neolacto-series gangliosides having linear poly-N-acetyl-lactosaminyl oligosaccharide structure have been demonstrated to be increased characteristically during granulocytic differentiation of human promyelocytic leukemia cell line HL-60 cells induced by dimethyl sulfoxide or retinoic acid (Nojiri, H., Takaku, F., Tetsuka, T., Motoyoshi, K., Miura, Y., and Saito, M. (1984) Blood 64, 534-541). When HL-60 cells were cultured in the presence of neolacto-series gangliosides prepared from mature granulocytes, the cells were found to be differentiated into mature granulocytes on the basis of the changes of morphology, surface membrane antigens, nonspecific esterase activity, and the activity of phagocytosis and respiratory burst. The differentiation of cells was dependent on the concentration of gangliosides and accompanied with inhibition of cell growth. These findings suggest that the particular ganglioside molecules play an important role in regulation of cell differentiation and that the appearance of neolacto-series gangliosides on cell surface membrane not only triggers the differentiation but also determines the direction of differentiation in HL-60 cells.