Effect of changes in the level of light harvesting complexes of Rhodobacter sphaeroides on the photoheterotrophic production of hydrogen
Effect of changes in the level of light harvesting complexes of Rhodobacter sphaeroides on the photoheterotrophic production of hydrogen
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DOI：
10.1016/j.ijhydene.2005.04.053
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发表时间：
2006-03-01
影响因子：
7.2
通讯作者：
Lee, JK
中科院分区：
文献类型：
--
作者：
Kim, EJ;Kim, JS;Lee, JK
增加光合光谱复合物的水平，通过维持质粒携带的DNA编码puhA，pufBA，或pucBAC在反式中的球形红细菌导致减少的光异养生产的H-2。然而，去除B875或B800-850捕光（LH）复合物对H-2产生的影响不同。缺失B875复合物后的pufBA（突变体PUF 1）的框内删除不仅减缓了光合异养生长，但也减少了H2的生产，表明LH过程的复合物的基本要求。然而，pucBA缺失的突变体PUC 1缺乏B800-850复合物，与其亲本细胞相比，增加了H-2的产量约两倍，给予光照射（10 W/m2）饱和野生型的生长。PUC 1的H-2产量没有与光强度成比例地增加，这在野生型中也观察到。因此，我们认为，在本工作中采用的实验条件下，光不限于HI生产的细胞，但用于形成B800-850复合物的细胞能量可能流入代谢，导致H-2生产PUC 1。(c)2005年国际氢能协会。由爱思唯尔有限公司出版。保留所有权利。
Increase in levels of photosynthetic spectral complexes by maintaining the plasmids harboring DNA encoding puhA, pufBA, or pucBAC in trans in Rhodobacter sphaeroides resulted in decrease of the photoheterotrophic production of H-2. However, removal of B875 or B800-850 light-harvesting (LH) complexes affected H-2 production differently. Lack of B875 complex following in-frame deletion of pufBA (mutant PUF1) not only slowed photoheterotrophic growth but also decreased H2 production, indicative of the essential requirement of the complex for LH process. However, the pucBA-deleted mutant, PUC1 lacking of B800-850 complex, increased H-2 production in comparison with its parental cell by approximately twofold, given irradiated with light (10W/m(2)) saturating the growth of wild type. The H-2 production of PUC1 did not increase in proportion to the light intensity, which is also observed with wild type. Thus, we suggest that light is not limited for the HI production of the cells under the experimental conditions employed in this work, but the cellular energy to be used for the formation of B800-850 complex may flow into the metabolism leading to the H-2 production in PUC1. (c) 2005 International Association for Hydrogen Energy. Published by Elsevier Ltd. All rights reserved.