STRATEGY FOR EFFICIENT DETECTION OF RESPIRATORY VIRUSES IN PEDIATRIC CLINICAL SPECIMENS
STRATEGY FOR EFFICIENT DETECTION OF RESPIRATORY VIRUSES IN PEDIATRIC CLINICAL SPECIMENS
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DOI：
10.1016/0732-8893(86)90033-7
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发表时间：
1986-11-01
影响因子：
2.9
通讯作者：
RIVETNA, KA
中科院分区：
文献类型：
--
作者：
ARENS, MQ;SWIERKOSZ, EM;RIVETNA, KA
在 1982-1983 年 RSV 季节（155 个样本）中，使用多克隆呼吸道合胞病毒 (RSV) 特异性抗体制剂进行直接免疫荧光 (IF) 进行抗原检测，与病毒培养相比，总体灵敏度为 94%，特异性为 87%。间接 IF 在 1983-1984 年季节（265 个样本）使用，表现出 96% 的敏感性和 79% 的特异性。在这两个季节中，224 个 RSV IF 阴性样本中的 42 个 (18.8%) 生长了 RSV 以外的病毒。 1984-1985年冬季，我们用IF法对297份标本进行了RSV筛查，80份（27%）呈阳性。 44 份 (20%) IF 阴性样本对 RSV(2) 或其他病毒(44) 培养呈阳性。我们的结论是，为了降低成本和快速检测呼吸道病毒，一旦配备适当的实验室完全熟悉 IF 技术，就可以通过 IF 筛查儿科呼吸道标本中的 RSV，并且只有 Phe IF 阴性标本需要在冬季呼吸道季节接种到细胞培养物中以分离病毒。
Direct immunofluorescence (IF) with a polyclonal respiratory syncytial virus (RSV)-specific antibody preparation was used for antigen detection during the 1982-1983 RSV season (155 specimens) and gave an overall sensitivity of 94% with 87% specificity compared with viral culture. Indirect IF was used in the 1983-1984 season (265 specimens) and exhibited sensitivity of 96% with specificity of 79%. During these two seasons, 42 of 224 (18.8%) specimens that were IF-negative for RSV grew viruses other than RSV. In the winter of 1984-1985, we screened 297 specimens for RSV by IF and 80(27%) were positive. Forty-four (20%) of the IF-negative specimens were culture-positive for RSV(2) or other viruses(44). We conclude that, in the interest of cost reduction and expeditious detection of respiratory viruses, once a properly equipped laboratory has become thoroughly familiar with IF techniques, pediatric respiratory specimens can be screened for RSV by IF and only Phe IF-negative specimens need be inoculated into cell cultures for isolation of virus during the winter respiratory season.