HUMAN-COLON CELLS - CULTURE AND INVITRO TRANSFORMATION
HUMAN-COLON CELLS - CULTURE AND INVITRO TRANSFORMATION
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DOI：
10.1126/science.6328655
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发表时间：
1984-01-01
期刊：
影响因子：
56.9
通讯作者：
AUST, JB
中科院分区：
文献类型：
--
作者：
MOYER, MP;AUST, JB
体外培养正常人结肠粘膜上皮细胞，并用致瘤猿猴DNA病毒（SV40）和化学致癌物氧化偶氮甲烷处理。SV40和氧化偶氮甲烷都改变了正常人结肠细胞的许多表型特征，包括它们的形态、培养寿命、在软琼脂中的生长、底物粘附和花生凝集素结合。SV40转化子合成核内T抗原。正常人结肠粘膜细胞明显向恶性表型转化。
Normal human colon mucosal epithelial cells were cultured in vitro and treated with the oncogenic simian DNA virus (SV40) and the chemical carcinogen azoxymethane. Both SV40 and azoxymethane altered a number of phenotypic characteristics of the normal human colon cells, including their morphology, culture longevity, growth in soft agar, substrate adherence and peanut agglutinin binding. The SV40 transformants synthesized intranuclear T antigen. Normal human colon mucosal cells were apparently transformed toward the malignant phenotype.