Yersinia pseudotuberculosis disseminates directly from a replicating bacterial pool in the intestine
Yersinia pseudotuberculosis disseminates directly from a replicating bacterial pool in the intestine
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DOI：
10.1084/jem.20060905
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发表时间：
2006-06-12
影响因子：
15.3
通讯作者：
Isberg, Ralph R.
中科院分区：
文献类型：
--
作者：
Barnes, Penelope D.;Bergman, Molly A.;Isberg, Ralph R.
分析了口服接种假结核耶尔森菌在小鼠体内的传播情况。口服接种后不久，假结核杆菌转移到肝脏和脾脏等器官，但很快被清除。相比之下，第二次暂时不同的细菌易位事件导致细菌的成功肝脾复制。可以在缺乏Peyer补丁的小鼠突变体的这些器官中建立复制的细菌池。这些动物经常有不育的肠系膜淋巴结，这一发现与易位发生独立于区域淋巴结定植一致。与公认的肠道病原体传播模型进一步矛盾的是，克隆分析显示，C57BL/6J小鼠脾脏和肝脏的致病细菌来源于肠道淋巴结外的群体。易位前肠道内细菌的复制似乎对传播至关重要，因为链霉素对肠道细菌生长的短暂选择性抑制延迟了假结核杆菌的传播。这些结果共同表明，肝脾定植似乎与假结核杆菌在肠腔内成功建立复制的能力密切相关，而不是在播散之前从肠道到区域淋巴结的有序传播。
Dissemination of Yersinia pseudotuberculosis within mice after oral inoculation was analyzed. Y. pseudotuberculosis translocated to organs such as the liver and spleen shortly after oral inoculation, but was quickly cleared. In contrast, a second temporally distinct bacterial translocation event resulted in successful hepatosplenic replication of the bacteria. Replicating pools of bacteria could be established in these organs in mouse mutants that lacked Peyer's patches. These animals frequently had sterile mesenteric lymph nodes, a finding consistent with translocation taking place independently of regional lymph node colonization. In further contradiction to accepted models for dissemination of enteropathogens, clonal analysis revealed that bacteria causing disease in the spleen and liver of C57BL/6J mice were derived from populations located outside the intestinal lymph nodes. Replication of bacteria in the intestine before translocation appeared critical for dissemination, as transient selective suppression by streptomycin of bacterial growth in the intestine delayed dissemination of Y. pseudotuberculosis. These results collectively indicate that hepatosplenic colonization appears intimately connected with the ability of Y. pseudotuberculosis to successfully establish replication in the intestinal lumen and does not result from ordered spread leading from the intestine to regional lymph nodes before dissemination.