Molecular phylogeny of Dipterocarpaceae in Southeast Asia using RFLP of PCR-amplified chloroplast genes
Molecular phylogeny of Dipterocarpaceae in Southeast Asia using RFLP of PCR-amplified chloroplast genes
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DOI：
10.1007/bf00225722
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发表时间：
1996-07
影响因子：
5.4
通讯作者：
Y. Tsumura;T. Kawahara;R. Wickneswari;K. Yoshimura
中科院分区：
文献类型：
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作者：
Y. Tsumura;T. Kawahara;R. Wickneswari;K. Yoshimura
龙脑香科（Dipterocarpaceae）是东南亚顶极热带雨林地区的优势科，它包含了该地区最重要的商业木材物种。利用聚合酶链反应扩增叶绿体DNA特异基因的限制性片段长度多态性，构建了龙脑香科龙脑香亚科的分子系统学。在10个属30个种中，共检测到141个位点的变化，涉及11个基因：rbcL、psbA、psbD、rpoB、rpoC、petB、atpH、16 S、psaA、petA和trnK。以乌普那为外类群，采用Wanger简约法和邻接法构建系统发育树，结果显示乌普那为5个单系类群，分别为：HopeaShorea-Parashorea-Neobalanocarpus、Dryobalanops、Dipterocarpus、Anisoptera-Vatica-Cotylelobium和乌普那。系统发育树清楚地将具有两个不同基本染色体数目的物种分开：第一组为isx=7，另一组为isx=11。其中x =7的类群被认为处于一个共形特征状态，拟沙枣属是大多数种的姊妹种，新栎属和霍普属是两个种的姊妹支，而紫堇属和梵蒂冈属是近缘种。我们的结论与木材解剖数据分析得出的分类学结果一致，也与Symington和Ashton的分类学分类一致。
Dipterocarpaceae is the dominant family of Southeast Asia's climax tropical rain forest region, and it contains the region's most important commercial timber species. A molecular phylogeny of the Dipterocarpaceae subfamily Dipterocapoideae was constructed using restriction fragment length polymorphisms of polymerase chain reaction-amplified specific genes in chloroplast DNA. A total of 141 site changes were detected among ten genera and 30 species in 11 different genes:rbcL,psbA,psbD,rpoB,rpoC,petB,atpH, 16S,psaA,petA andtrnK. Phylogenetic trees constructed by Wanger parsimony and neighbor-joining methods, usingUpunaas the outgroup, displayed five monophytelic groups that includedUpuna: HopeaShorea-Parashorea-Neobalanocarpus; Dryobalanops; Dipterocarpus; Anisoptera-Vatica-Cotylelobium; andUpuna. The phylogenetic trees clearly separate species with two different base chromosome numbers: the first group isx=7, and the other isx=11. Thex=7 group is thought to be in a synapomorphic character state.Parashorea lucidais a sister to mostShoreaspecies.Neobalanocarpus heimiiandHopeafrom a clade of a sister to twoShoreaspecies, andCotylelobiumandVaticaare closely related species. Our conclusions agree with a phylogeny derived from wood anatomy data analysis, and with Symington's and Ashton's taxonomic classifications.