Sensory systems and ionocytes are targets for silver nanoparticle effects in fish.
Sensory systems and ionocytes are targets for silver nanoparticle effects in fish.
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感觉系统和离子细胞是银纳米粒子对鱼类产生影响的目标。
DOI：
10.1080/17435390.2016.1206147
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发表时间：
2016
期刊：
影响因子：
5
通讯作者：
Osborne OJ
中科院分区：
文献类型：
--
作者：
Osborne OJ
一些纳米颗粒（NPs）可能会在暴露的生物体中引起不良健康影响，但迄今为止，野生动物中的证据非常有限。银纳米颗粒（AgNP）在与某些环境暴露相关的浓度下可能对水生生物（包括鱼类）有毒。我们应用全膜原位杂交（WISH）技术在斑马鱼胚胎和幼体中检测了一系列与解毒过程和氧化应激有关的基因，包括金属硫蛋白（mt 2）、谷胱甘肽S-转移酶pi（gstp）、谷胱甘肽S-转移酶mu（gstm 1）、血红素加氧酶1和铁蛋白重链1与本体对应物和离子银（AgNO 3）相比，鉴定AgNPs的潜在靶组织和作用机制。AgNPs可上调mt 2、gstpandgstm 1的表达，下调hmox 1和fth 1的表达，并具有生命阶段特异性和组织特异性。反应组织包括嗅球、侧线神经瘤和皮肤中的离子细胞，可能对嗅觉、行为和离子平衡的维持产生影响。银离子诱导与AgNP相似的基因反应并影响相同的靶组织。与涂覆的AgNP相比，AgNP引起的靶基因应答水平更类似于银处理，表明所见的应答是由于释放的银离子。在Nrf 2突变体斑马鱼中，mt 2（24 hpf）和gstp（3 dpf）的表达与野生型斑马鱼相比在暴露于AgNPs时不可检测或处于较低水平，表明这些基因反应通过Nrf 2-Keap途径控制。
Some nanoparticles (NPs) may induce adverse health effects in exposed organisms, but to date the evidence for this in wildlife is very limited. Silver nanoparticles (AgNPs) can be toxic to aquatic organisms, including fish, at concentrations relevant for some environmental exposures. We applied whole mountin-situhybridisation (WISH) in zebrafish embryos and larvae for a suite of genes involved with detoxifying processes and oxidative stress, including metallothionein (mt2), glutathionineS-transferase pi (gstp), glutathionineS-transferase mu (gstm1), haem oxygenase (hmox1) and ferritin heavy chain 1 (fth1) to identify potential target tissues and effect mechanisms of AgNPs compared with a bulk counterpart and ionic silver (AgNO3). AgNPs caused upregulation in the expression ofmt2, gstpandgstm1and down regulation of expression of bothhmox1andfth1and there were both life stage and tissue-specific responses. Responding tissues included olfactory bulbs, lateral line neuromasts and ionocytes in the skin with the potential for effects on olfaction, behaviour and maintenance of ion balance. Silver ions induced similar gene responses and affected the same target tissues as AgNPs. AgNPs invoked levels of target gene responses more similar to silver treatments compared to coated AgNPs indicating the responses seen were due to released silver ions. In theNrf2zebrafish mutant, expression ofmt2(24 hpf) andgstp(3 dpf) were either non-detectable or were at lower levels compared with wild type zebrafish for exposures to AgNPs, indicating that these gene responses are controlled through the Nrf2-Keap pathway.