ACCELERATED AUTOMATIC CHROMATOGRAPHIC ANALYSIS OF AMINO ACIDS ON A SPHERICAL RESIN.
ACCELERATED AUTOMATIC CHROMATOGRAPHIC ANALYSIS OF AMINO ACIDS ON A SPHERICAL RESIN.
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球形树脂上氨基酸的加速自动色谱分析。
DOI：
10.1021/ac60228a008
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发表时间：
1965
影响因子：
7.4
通讯作者：
J. A. Patterson
中科院分区：
文献类型：
--
作者：
J. V. Benson;J. A. Patterson
在Beckman氨基酸分析仪中，通过使用球形颗粒树脂，加速了离子交换色谱法对氨基酸的分析。酸性和中性氨基酸在57 × 0.90 cm的色谱柱上进行分析。树脂柱，缓冲液体积输入为68 ml。小时（线性流速为106 cm./小时）。碱性氨基酸在5.0 × 0.90 cm的分析仪上进行分析。树脂柱在相同的缓冲液流速。该系统将总分析时间缩短至4小时5分钟（酸性和中性氨基酸为3小时10分钟，碱性氨基酸为55分钟）。通过正确的分析顺序，可以在8小时内完成三次分析。
Acceleration of analysis of amino acids by ion exchange chromatography has been facilitated in the Beckman amino acid analyzer by the use of a spherical particle resin. The acidic and neutral amino acids are analyzed on a 57-by 0.90-cm. resin column at a buffer volumetric input of 68 ml./hour (linear flow rate of 106 cm./hour). The basic amino acids are analyzed on a 5.0-by 0.90-cm. resin column at the same buffer flow rate. This system reduces the total analysis time to 4 hours, 5 minutes;(3 hours, 10 minutes for the acidic and neutral amino acids and 55 minutes for the basic amino acids). With the proper analysissequence, three analyses can be completed in an 8-hour day.